I-Cyclohexanone monooxygenase (CHMO)

Incazelo emfushane:

Mayelana ne-Cyclohexanone monooxygenase

I-ES-CHMO (Cyclohexanone monooxygenase): i-Baeyer Villiger monooxygenase (BVMO) ene-cyclohexanone njenge-substrate, inga-oxidize i-cyclohexanone ku-cyclohexanol isebenzisa umoya-mpilo.

Iwuhlobo lwe-enzyme encike ku-flavin.I-flavin ene-oxidized iyancipha ibe yi-flavin ngomjikelezo wokunciphisa i-coenzyme II (NADPH) ye-oxidation elandelayo.Ngakho-ke, i-cofactor NADPH iyadingeka kumjikelezo we-flavin.Kunohlobo olungu-1 kuphela lomkhiqizo we-enzyme ye-CHMO (Inombolo njengokuthi ES-CHMO101) ethuthukiswe yi-SyncoZymes, engasetshenziswa ukwenza i-cyclohexanone ibe yi-cyclohexanone.

Uhlobo lokusabela kwe-Catalytic:

I-Cyclohexanone monooxygenase CHMO1

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Ukucaciswa
Ikhithi Yokuhlola (SynKit)	I-ES-CHMO-101	isethi ye-1 Ketoreductases, 1 mg into ngayinye engu-1 * 1mg / into

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.

Imiyalo yokusebenzisa:

➢ Ngokuvamile, isistimu yokusabela kufanele ihlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-coenzyme kanye nohlelo lokuvuselela i-coenzyme (isb. i-glucose ne-glucose dehydrogenase).
➢ I-CHMO kufanele yengezwe okokugcina ohlelweni lokusabela, ngemva kokuthi i-pH nezinga lokushisa kulungiswe esimweni sokusabela.

Izibonelo Zohlelo Lokusebenza:

Isibonelo 1(Ukwenziwa kwe-cyclohexanol kusuka ku-cyclohexanol)⁽¹⁾:

Isitoreji:

Gcina iminyaka emi-2 ngaphansi -20 ℃.

Qaphela:

Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.