I-Nitrile reductase (NRED)
Incazelo emfushane:
Mayelana ne-Nitrile reductase
Kukhona uhlobo olu-3 lomkhiqizo we-enzyme ye-NRED (Inombolo njenge-ES-NRED101~ ES-NRED103) eyakhiwe yi-SyncoZymes.I-NRED ingadala ukwehliswa kweqembu le-nitrile libe yi-amine.Enqubweni ye-catalysis, i-NADPH iyadingeka njengesithuthi se-hydrogen.
Uhlobo lokusabela lwe-Nitrile reductasecatalytic
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
| Ukucaciswa | ES- NRED -101~ ES- NRED -103 | isethi ye-3 Nitrile Reductase, 50 mg ngayinye izinto ezi-3 * 50mg / into, noma elinye inani |
Isistimu yokusabela elandelayo ingasetshenziswa njengereferensi:
I-Substrate engu-1-10 mg/ml
10 mg/ml i-enzyme
10% (v/v) i-Organic cosolvent (1)
100 mM Ibhafa ye-Phosphate (pH7.0)
0.2 mg/ml NADP+
10 mg/ml iGlucose
2 mg/ml iGlucose dehydrogenase (GDH)
Fukamisa ukusabela endaweni enyakazisa izinga lokushisa engashintshi (isibonelo, 30oC, 150rpm) amahora angama-24~48.I-Centrifuge ingxube ngayinye ukuze iphuze amaprotheni, wona angaphezu kwamandla angasetshenziselwa ukuhlaziya i-TLC, HPLC noma i-GC.
Qaphela (1): Isinyibilikisi se-Organic (njenge-DMSO, i-methanol, i-acetonitrile, i-IPA, njll.) iyakhethwa, isetshenziselwa ama-substrates angancibiliki.
