I-Amine Dehydrogenase (AmDH)

Incazelo emfushane:

Mayelana ne-Amine Dehydrogenase

I-ES-AmDH (i-Amine Dehydrogenase): i-enzyme engakwazi ukuvuselela i-ammoniation yeqembu le-carbonyl futhi iyinciphise ibe yiqembu lama-amino.Kuyi-coenzyme encike ku-NADH, futhi i-NADH isebenza njengesidluliseli se-hydrogen.Kunezinhlobo ezi-4 zemikhiqizo ye-enzayimu ye-AmDH (Inombolo njengokuthi ES-AmDH101～ES-AmDH104) ethuthukiswe yi-SyncoZymes, engasetshenziswa ku-regioselective noma i-stereoelective reductive amination yama-aldehydes nama-ketone.

Uhlobo lokusabela kwe-Catalytic:

Mayelana ne-Amine Dehydrogenase2

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ulwazi Lomkhiqizo:

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.

Imiyalo yokusebenzisa:

➢ Ngokuvamile, isistimu yokusabela kufanele ihlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-coenzyme kanye nohlelo lokuvuselela i-coenzyme (isb. i-glucose ne-glucose dehydrogenase).
➢ I-AmDH kufanele yengezwe okokugcina ohlelweni lokusabela, ngemuva kokuthi i-pH nezinga lokushisa kulungiswe kusimo sokusabela.

Izibonelo Zohlelo Lokusebenza:

Isibonelo 1(Ukwenziwa kwama-chiral amines ahambisanayo ngokuncishiswa kwe-alkyl aryl ketones)⁽¹⁾:

Isitoreji:

Gcina iminyaka emi-2 ngaphansi -20 ℃.

Qaphela:

Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.