Isithombe Esifakiwe se-Cyclohexanone monooxygenase (CHMO)

I-Cyclohexanone monooxygenase (CHMO)

Incazelo emfushane:

Mayelana ne-Cyclohexanone monooxygenase

I-ES-CHMO (i-Cyclohexanone monooxygenase): i-Baeyer Villiger monooxygenase (BVMO) ene-cyclohexanone njenge-substrate, ingaxuba i-cyclohexanone ibe yi-cyclohexanol isebenzisa i-oxygen.

Luhlobo lwe-enzyme encike ku-flavin. I-flavin e-oxidized incishiswa ibe yi-flavin ngomjikelezo wokunciphisa i-coenzyme II (NADPH) ukuze kutholakale i-oxidation elandelayo. Ngakho-ke, i-cofactor NADPH iyadingeka emjikelezweni we-flavin. Kukhona uhlobo olulodwa kuphela lomkhiqizo we-enzyme ye-CHMO (Inombolo njenge-ES-CHMO101) eyakhiwa yi-SyncoZymes, engasetshenziswa ukuxubha i-cyclohexanone ibe yi-cyclohexanone.

Uhlobo lokusabela kwe-Catalytic:

I-Cyclohexanone monooxygenase CHMO1

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Imininingwane
Ikhithi Yokuhlola (i-SynKit)	I-ES-CHMO-101	isethi yama-Ketoreductases angu-1, 1 mg ngayinye 1 into * 1mg/into

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-coenzyme kanye nohlelo lokuvuselela i-coenzyme (isb. i-glucose kanye ne-glucose dehydrogenase).
➢ I-CHMO kufanele ifakwe ekugcineni ohlelweni lokusabela, ngemuva kokuthi i-pH kanye nokushisa kulungiswe esimweni sokusabela.

Izibonelo Zokusetshenziswa:

Isibonelo 1 (Ukwenziwa kwe-cyclohexanol kusuka ku-cyclohexanol)⁽¹⁾:

Isitoreji:

Gcina iminyaka emibili ngaphansi kuka -20℃.

Ukunakwa:

Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.