I-Phenylalanine dehydrogenase (PDH)
Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
I-Enzyme Powder | ES-PDH-101~ ES-PDH-108 | isethi ye-8 Nitrile Reductase, 50 mg ngayinye 8 izinto * 50mg / into, noma elinye inani |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ihlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-coenzyme (NAD(H)), uhlelo lokuvuselela i-coenzyme (isb. i-ammonium formate ne-formate dehydrogenase).
➢ Uhlobo ngalunye lwe-ES-PDH oluhambisana nezimo ezihlukahlukene zokusabela, ezingafundwa ngazinye uma kudingeka.
➢ I-ES-PDH kufanele yengezwe okokugcina ohlelweni lokusabela ukuze kugcinwe umsebenzi.
Isibonelo 1(I-Biosynthesis ye-phenylalanine derivatives esuka kokuphuma kokunye kwe-phenylpyruvate)(1):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. UFrancesca Paradisi, uStuart Collins, et al.Ijenali ye-Biotechnology, 2007, 128, 408–411.