I-Epoxide hydrolase (EH)
Incazelo emfushane:
Mayelana ne-epoxide hydrolase
I-EH yi-ether hydrolase evuselela i-stereoselective hydrolysis yama-epoxide e-racemic ukukhiqiza ama-epoxide angu-1, 2-diol kanye nama-epoxide asebenzayo, angasetshenziswa ekuxazululeni ama-epoxide e-chiral noma ekulungiseleleni ama-diols e-chiral. Asetshenziswa kabanzi kwezokwelapha nakweminye imikhakha. Kunezinhlobo ezintathu zemikhiqizo yama-epoxide hydrolases (Inombolo njenge-ES-EH-101～ES-EH-103) eyathuthukiswa yi-SyncoZymes. I-ES-EH iyithuluzi eliwusizo lokuvuselela ukwenziwa kwama-alcohols e-chiral ngokusebenzisa i-stereoselective hydrolysis yama-epoxide e-aliphatic ne-aromatic.
Uhlobo lokusabela kwe-Catalytic:
Iselula/I-Wechat/I-WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | ES-EH-101~ ES-EH-103 | isethi yama-Epoxide Hydrolases amathathu, 50 mg ngalinye Izinto ezi-3 * 50mg / into, noma olunye ubuningi |
| Ikhithi Yokuhlola (i-SynKit) | I-ES-EH-300 | isethi yama-Epoxide Hydrolases amathathu, 1 mg ngayinye Izinto ezi-3 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer (i-pH yokusabela efanele) kanye ne-ES-EH.
➢ Zonke izinhlobo ze-ES-EH ezihambisana nezimo ezahlukahlukene zokusabela ezifanele kufanele zifundwe ngazinye.
➢ I-substrate eningi noma umkhiqizo one-concentration ephezulu ungavimbela umsebenzi we-ES-EH. Kodwa-ke, ukuvinjelwa kungancishiswa ngokufaka i-substrate ngenqwaba.
Isibonelo 1(1):
Gcina iminyaka emibili ngaphansi kuka -20℃.
Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
1. Kim YH, Lee I, Choi SH, e tal. J Mol Catal B-Enzym, 2013, 89: 48-51.
