I-Amidase (AMD)
Ama-Enzymes:Ama-macromolecular biological catalysts, ama-enzyme amaningi angamaprotheni
I-Amidase:Catalyze the hydrolysis of endogenous and foreign aliphatic and aromatics amides by transfering acyl group to water with production of free acids and ammonia.Ama-Hydroxamic acids namanye ama-asidi e-organic asetshenziswa kakhulu njengezidakamizwa ngoba ayizici zokukhula, ama-antibiotics kanye nama-tumor inhibitors.Ama-amidase angahlukaniswa abe uhlobo lwe-R kanye nohlobo lwe-acylases ngokusho kwe-catalyst stereoselectivity.
Ngaphezu kokwenza i-hydrolysis yama-amide, i-amidase ingaphinda idale ukusabela kokudluliselwa kwe-acyl lapho kukhona ama-co-substrates afana ne-hydroxylamine.
I-Amidase enemithombo ehlukene inokucaciswa kwe-substrate okuhlukile, eminye yayo ikwazi ukwenza i-hydrolyze kuphela ama-amide anephunga elimnandi, eminye yayo ingakwazi ukwenza i-hydrolyze kuphela ama-aliphatic amide, kanti enye i-hydrolyze α-noma ω-amino amide.Iningi lama-amine anomsebenzi omuhle we-catalytic kuphela wama-acyclic noma ama-amide anephunga elimnandi, kodwa kuma-aromatics ayinkimbinkimbi, ama-heterocyclic amide, ikakhulukazi ama-amide ane-ortho substituents, ngokuvamile awasebenzi kakhulu (ama-enzyme ambalwa kuphela abonisa imiphumela engcono ye-catalytic).
Indlela ye-Catalytic:
| Ama-enzyme | Ikhodi Yomkhiqizo | Ikhodi Yomkhiqizo |
| I-Enzyme Powder | ES-AMD-101~ ES-AMD-119 | isethi 19 amidases, 50 mg ngayinye 19 izinto * 50mg / into, noma omunye ubuningi |
| Ikhithi Yokuhlola (SynKit) | ES-AMD-1900 | isethi 19 amidases, 1 mg ngayinye 19 izinto * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ High ukuguqulwa ukusebenza kahle.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ukuhlolwa kwe-enzyme kufanele kwenziwe kuma-substrate athile ngenxa yokucaciswa kwe-substrate, futhi uthole i-enzyme eyenza i-substrate eqondiwe ibe nomthelela omuhle kakhulu we-catalytic.
➢ Ungalokothi uthintane nezimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-organic solvent enokugxila okuphezulu.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo se-buffer (I-pH yokusabela efanele ye-enzyme).Ama-co-substrates afana ne-hydroxylamine kufanele abe khona ohlelweni lokusabela lokudluliswa kwe-acyl.
➢ I-AMD kufanele yengezwe okokugcina ohlelweni lokusabela olune-pH esezingeni eliphezulu kanye nezinga lokushisa.
➢ Zonke izinhlobo ze-AMD zinemibandela ehlukahlukene yokusabela kahle, ngakho ngayinye yazo kufanele iqhutshekwe ifundwe ngayodwa.
Isibonelo 1(1):
Umsebenzi we-Hydrolysis kuma-Amide Substrates ahlukene
| I-substrate | Umsebenzi othize imiz min-1mg-1 | I-substrate | Umsebenzi othize imiz min-1mg-1 |
| I-acetamide | 3.8 | ο-OH benzamide | 1.4 |
| I-Propionamide | 3.9 | p-OH benzamide | 1.2 |
| I-Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| I-Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο- Toluamide | 0.3 |
| I-Pentanamide | 22.0 | p- Toluamide | 8.1 |
| I-Hexanamide | 6.4 | I-Nicotinamide | 1.7 |
| I-Cyclohexanamide | 19.5 | I-Isonicotinamide | 1.8 |
| I-Acrylamide | 10.2 | I-Picolinamide | 2.1 |
| I-Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| I-Prolinamide | 3.4 | I-Indol-3-acetamide | 1.9 |
| I-Benzamide | 6.8 |
Ukusabela kwenziwa kusixazululo sebhafa se-sodium phosphate 50mM, pH 7.5, ku-70 ℃.
| Ama-Amide | IHydroxylamine | IHydrazine |
| I-acetamide | 8.4 | 1.4 |
| I-Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| I-Benzamide | 9.2 | 6.1 |
Ukusabela kwenziwa kusixazululo sebhafa se-sodium phosphate 50mM, pH 7.5, ku-70 ℃.
Ukugxiliswa kwe-reagent ehlobene: ama-amide, 100 mM(benzamide, 10 mM);i-hydroxylamine ne-hydrazine, 400 mM;I-enzyme 0.9 μM.
Isibonelo sesi-2(2):
Isibonelo sesi-3(3):
1. D'Abusco AS, Ammendola S., et al.I-Extremophiles, 2001, 5:183-192.
2. I-Guo FM, i-Wu JP, i-Yang LR, njll.Inqubo ye-Biochemistry, 2015, 50(8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, et al.I-Bioorganic Chemistry, 2017, Itholakala ku-inthanethi 7.
