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I-SyncoZymes

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Isithombe Esifakiwe se-Esterase ne-Lipase (PLE ne-CALB)
  • I-Esterase ne-Lipase (PLE ne-CALB)
  • I-Esterase ne-Lipase (PLE ne-CALB)

I-Esterase ne-Lipase (PLE ne-CALB)

Incazelo emfushane:

Mayelana ne-Esterase ne-Lipase

Ama-ES-PLE: Uhlobo lwama-hydrolase akhuthaza ukwakheka nokuqhekeka kwama-ester bond. Angaba yingxenye yezinhlobo eziningi zokusabela kwamakhemikhali, njengokwenziwa kwe-ester, ukuguqulwa kwe-ester kanye ne-hydrolysis. Asetshenziswa kabanzi emikhakheni eminingi efana nokucubungula ukudla, imboni yamakhemikhali, i-biomedicine, imithi ye-chiral kanye nokwelashwa kwemvelo.

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com


Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Mayelana ne-Esterase ne-Lipase:

Kunezinhlobo ezingu-26 zemikhiqizo ye-enzyme ye-PLE (Inombolo njenge-ES-PLE-101～ES-PLE-126) eyathuthukiswa yi-SyncoZymes. I-ES-PLE ingasetshenziswa ekufakweni kwe-hydrolysis yamakhemikhali e-aliphatic ne-ester, noma isixazululo se-regioselective kanye ne-stereoselective ukuhlanganisa ama-chiral acid kanye ne-derivatives yawo.

Uhlobo lokusabela kwe-Catalytic:

I-Esterase ne-Lipase (PLE ne-CALB)
I-Esterase ne-Lipase (PLE ne-CALB)1

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer kanye ne-ES-PLE. Ukuqinisekiswa kwe-esterification kwamanye ama-ES-PLE kwenziwa esigabeni se-organic.
➢ Zonke izinhlobo ze-ES-PLE ezihambisana nezimo ezahlukahlukene zokusabela ezifanele kufanele zifundwe ngazinye.

Izibonelo Zokusetshenziswa:

Isibonelo 1 (Ukwenziwa kwe-Biosynthesis ye-Pregabalin Intermediate)(1):

i-calb1

Isibonelo 2(2):

i-calb2

Isibonelo 3(3):

i-calb3

Isibonelo 4(4):

i-calb5

Isitoreji:

Gcina iminyaka emibili ngaphansi kuka -20℃.

Ukunakwa:

Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi.

Izinkomba:

1. Xu FX, Chen SY, Xu G, etal. I-Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal. J. Am. I-Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. USynlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917

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