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SyncoZymes

imikhiqizo

I-Esterase&Lipase (PLE&CALB) Isithombe Esifakiwe
  • I-Esterase&Lipase (PLE&CALB)
  • I-Esterase&Lipase (PLE&CALB)

I-Esterase&Lipase (PLE&CALB)

Incazelo emfushane:

Mayelana ne-Esterase&Lipase

ES-PLEs: Ikilasi lama-hydrolases abangela ukwakheka nokuphuka kwamabhondi e-ester.Bangabamba iqhaza ezinhlotsheni eziningi zokusabela kwamakhemikhali ezinto eziphilayo, njenge-esterification, i-transesterification kanye ne-hydrolysis.Zisetshenziswa kakhulu emikhakheni eminingi efana nokucubungula ukudla, imboni yamakhemikhali, i-biomedicine, imithi ye-chiral kanye nokwelashwa kwemvelo.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Mayelana ne-Esterase&Lipase:

Kunezinhlobo ezingu-26 zemikhiqizo ye-enzyme ye-PLE (Inombolo njengokuthi ES-PLE-101～ES-PLE-126) ethuthukiswe yi-SyncoZymes.I-ES-PLE ingasetshenziselwa i-hydrolysis ye-aliphatic ne-ester compounds, noma ukulungiswa kwe-regioselective ne-stereoselective ukuze kuhlanganiswe ama-chiral acid kanye nokuphuma kwawo.

Uhlobo lokusabela kwe-Catalytic:

I-Esterase&Lipase (PLE&CALB)
I-Esterase&Lipase (PLE&CALB)1

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.

Imiyalo yokusebenzisa:

➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isisombululo se-buffer kanye ne-ES-PLE.Ukufakwa kwe-esterification kwamanye ama-ES-PLE kwenziwa esigabeni sezinto eziphilayo.
➢ Zonke izinhlobo ze-ES-PLE ezihambisana nezimo zokusabela ezifanele kufanele zifundwe ngazodwana.

Izibonelo Zohlelo Lokusebenza:

Isibonelo 1(I-Biosynthesis ye-Pregabalin Intermediate)(1):

calb1

Isibonelo sesi-2(2):

calb2

Isibonelo sesi-3(3):

calb3

Isibonelo sesi-4(4):

calb5

Isitoreji:

Gcina iminyaka emi-2 ngaphansi -20 ℃.

Qaphela:

Ungalokothi uthintane nezimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi.

Izithenjwa:

1. Xu FX, Chen SY, Xu G, e tal.Uhlelo lokusebenza.I-Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal.J. Am.I-Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.USynlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917

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