I-Alcohol oxidase (AOX)
Incazelo emfushane:
Mayelana ne-alcohol oxidase
I-ES-AOXs: Lawa ma-enzyme ayakwazi ukwenza i-oxidation yama-alcohols anamafutha noma ama-aryl-alcohols akhe ama-aldehydes.Basebenzisa i-oxygen yamangqamuzana kodwa ayidingi i-cofactor eyengeziwe, kanye ne-hydrogen peroxide ekhiqizwa.
Kunezinhlobo ezingu-6 zemikhiqizo ye-alcohol oxidases (Inombolo njengokuthi ES-AOX-101~ES-AOX-106) ethuthukiswe yi-SyncoZymes.I-ES-AOX101 kanye ne-ES-AOX102 bakhetha ama-aliphatic alcohol substrates, i-ES-AOX103~ ES-AOX105 ikhetha ama-alcohol substrates anephunga elimnandi, kanti i-ES-AOX106 i-cholesterol oxidase.I-SZ-AOX iyithuluzi eliwusizo lokwenza i-oxidation yotshwala obunamafutha noma i-aryl-alcohols ukukhiqiza ama-aldehydes.
Uhlobo lokusabela kwe-Catalytic:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
| I-Enzyme Powder | ES-AOX-101~ ES-AOX-105 | isethi 5 utshwala oxidase, 50 mg ngayinye 5 izinto * 50mg / into, noma omunye ubuningi |
| Ikhithi Yokuhlola (SynKit) | ES-AOX-500 | isethi 5 utshwala oxidase, 50 mg ngayinye 5 izinto * 50mg / into, noma omunye ubuningi |
★ Ukucaciswa okuphezulu kwe-substrate.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo se-buffer kanye ne-ES-AOX, futhi umoya-mpilo kufanele ube khona.
➢ Zonke izinhlobo ze-ES-AOX ezihambisana nezimo zokusabela ezinhle kakhulu, ezingafundwa ngazodwana.
➢ I-Substrate egxilile ephezulu noma umkhiqizo onawo ungase uvimbele umsebenzi we-ES-AOX.Kodwa-ke, ukuvimbela kungakhululwa ngokungezwa kwe-batch ye-substrate.
➢ Ukuqoqwa kwe-H2O2ohlelweni kuzoholela ekusebenzeni kwe-enzyme, engaxazululwa ngempumelelo ngokusetshenziswa kwe-catalase.
Isibonelo 1(I-Oxidation ye-aryl-alcohols)(1):
Isibonelo 2(I-Oxidation of fatty alcohols)(2):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. Benen J AE, Sa'nchez-Torres P, Wagemaker M JM, e tal.J Biol Chem, 1998, 273(14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal.I-Appl Microbiol Biot, 1992, 37: 66-73.
