I-Cytochrome P450 monooxygenase (CYP)

Incazelo emfushane:

Mayelana ne-CYP

I-ES-CYPs: Isigaba sama-enzyme e-redox ane-mercaptan-heme njengesikhungo esisebenzayo sokugqugquzela ukudluliswa komoyampilo.Ziyi-oxygenases yokugcina futhi idinga i-heme ukuze isebenze umoya-mpilo.

I-Cytochrome P450 monooxygenase yenza i-oxidation, i-epoxidation kanye ne-hydroxylation ye-alkyl, i-hydroxylation kanye ne-oxidation ye-ammonia, i-oxidation yesibabule, i-dealkylation (i-oksijeni, isulfure, i-ammonia), i-oxidative dehydrogenation, deamination and dehalogenation.
Kunezinhlobo ezingu-8 zemikhiqizo ye-cytochrome P450 monooxygenase (Inombolo njengokuthi ES-CYP-101～ES-CYP-108) ethuthukiswe yi-SyncoZymes.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Mayelana ne-CYP:

Uhlobo lokusabela kwe-Catalytic:

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhithi Yokuhlola (SynKit)	Ukucaciswa
I-Enzyme Powder	ES-CYP-101~ ES-CYP-108	isethi 8 Cytochrome P450 Monooxygenases, 50 mg ngayinye 8 izinto * 50mg / into, noma omunye ubuningi
Ikhithi Yokuhlola (SynKit)	ES-CYP-800	isethi ye-8 Cytochrome P450 Monooxygenases,1mg izinto eziyi-8 ngayinye * 1mg / into

Izinzuzo:

★ I-spectrum ye-substrate ebanzi.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.

Imiyalo yokusebenzisa:

➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo se-buffer (I-pH yokusabela ekahle ye-enzyme), i-coenzyme (NAD(H) noma i-NADP(H)), uhlelo lokuvuselela i-coenzyme (isb. i-glucose ne-glucose dehydrogenase) kanye ne-ES-CYP.i-coenzyme kanye nohlelo lokuvuselela i-coenzyme lungathathelwa indawo yi-hydrogen peroxide ohlelweni lokusabela ngokwengxenye.
➢ Zonke izinhlobo ze-ES-CYP ezihambisana nezimo zokusabela ezifanele kufanele zifundwe ngazodwana.
➢ I-Substrate egxilile ephezulu noma umkhiqizo onawo ungase uvimbele umsebenzi we-ES-CYP.Kodwa-ke, ukuvimbela kungakhululwa ngokungezwa kwe-batch ye-substrate.

Izibonelo Zohlelo Lokusebenza:

Isibonelo 1⁽¹⁾:

Isibonelo sesi-2⁽²⁾:

Isibonelo sesi-3⁽³⁾:

Isibonelo sesi-4⁽⁴⁾:

Isitoreji:

Gcina iminyaka emi-2 ngaphansi -20 ℃.

Qaphela:

Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.

Izithenjwa:

1. Zaretzki J, Matlock M, and Swamidass S JJ Chem.Inf.Imodeli, 2013, 53, 3373-3383.
2. Gannett P M., Kabulski J, Perez F A., e tal.J. Am.I-Chem.I-Soc., 2006, 128 (26), 8374–8375.
3. U-Cryle M J., uMatovic N J., no-De Voss J J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.I-Biochim.I-Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK e tal.I-Acta Crystallog.2009, 65, 372.