Isithombe Esifakiwe se-Cytochrome P450 monooxygenase (CYP)

I-Cytochrome P450 monooxygenase (CYP)

Incazelo emfushane:

Mayelana ne-CYP

Ama-ES-CYP: Uhlobo lwama-enzyme e-redox ane-mercaptan-heme njengesikhungo esisebenzayo sokuvuselela ukudluliselwa komoya-mpilo. Angama-oxygenase agcinayo futhi adinga i-heme ukuze asebenze umoya-mpilo.

I-Cytochrome P450 monooxygenase ivuselela ukujiya, i-epoxidation kanye ne-hydroxylation ye-alkyl, i-hydroxylation kanye ne-oxidation ye-ammonia, i-oxidation yesulfure, i-dealkylation (i-oxygen, i-sulfur, i-ammonia), i-oxidative dehydrogenation, i-deamination kanye ne-dehalogenation.
Kunezinhlobo ezingu-8 zemikhiqizo ye-cytochrome P450 monooxygenase (Inombolo njenge-ES-CYP-101～ES-CYP-108) eyenziwe yi-SyncoZymes.

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Mayelana ne-CYP:

Uhlobo lokusabela kwe-Catalytic:

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhithi Yokuhlola (i-SynKit)	Imininingwane
Impuphu ye-Enzyme	I-ES-CYP-101~ I-ES-CYP-108	isethi yama-Cytochrome P450 Monooxygenases angu-8, angu-50 mg ngalinye izinto ezingu-8 * 50mg / into, noma olunye ubuningi
Ikhithi Yokuhlola (i-SynKit)	I-ES-CYP-800	isethi yama-Cytochrome P450 Monooxygenases ayi-8, 1mg ngayinye Izinto eziyi-8 * 1mg / into

Izinzuzo:

★ I-spectrum ebanzi ye-substrate.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ngokuvamile, uhlelo lokusabela kufanele lufake i-substrate, isixazululo se-buffer (I-pH yokusabela okuhle kakhulu kwe-enzyme), i-coenzyme (i-NAD(H) noma i-NADP(H)), uhlelo lokuvuselela i-coenzyme (isb. i-glucose ne-glucose dehydrogenase) kanye nohlelo lokuvuselela i-coenzyme ne-coenzyme lwe-ES-CYP lungathathelwa indawo yi-hydrogen peroxide ohlelweni lokusabela oluyingxenye.
➢ Zonke izinhlobo ze-ES-CYP ezihambisana nezimo ezahlukahlukene zokusabela ezifanele kufanele zifundwe ngazinye.
➢ I-substrate ephezulu noma umkhiqizo one-concentration ungavimbela umsebenzi we-ES-CYP. Kodwa-ke, ukuvinjelwa kungancishiswa ngokungezwa kwe-substrate ngeqembu.

Izibonelo Zokusetshenziswa:

Isibonelo 1⁽¹⁾:

Isibonelo 2⁽²⁾:

Isibonelo 3⁽³⁾:

Isibonelo 4⁽⁴⁾:

Isitoreji:

Gcina iminyaka emibili ngaphansi kuka -20℃.

Ukunakwa:

Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.

Izinkomba:

1. UZaretzki J, uMatlock M, kanye noSwamidass S JJ Chem. Imodeli Yemininingwane, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal. J. Am. I-Chem. I-Soc., 2006, 128 (26), 8374–8375.
3. UCryle M J., uMatovic N J., noDe Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. I-Biochim. I-Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK e tal. I-Acta Crystallog. 2009, 65, 372.