Isithombe Esifakiwe se-Amine Dehydrogenase (AmDH)

I-Amine Dehydrogenase (AmDH)

Incazelo emfushane:

Mayelana ne-Amine Dehydrogenase

I-ES-AmDH (i-Amine Dehydrogenase): i-enzyme engakwazi ukuvuselela i-ammoniation yeqembu le-carbonyl futhi iyinciphise ibe yiqembu lama-amino. Iyi-enzyme encike ku-coenzyme NADH, futhi i-NADH isebenza njengomthumeli we-hydrogen. Kunezinhlobo ezine zemikhiqizo ye-enzyme ye-AmDH (Inombolo njenge-ES-AmDH101 ~ ES-AmDH104) eyenziwe yi-SyncoZymes, engasetshenziswa ekuhlanganiseni ama-aldehydes nama-ketone ngokukhetha kabusha noma ngokukhetha kabusha.

Uhlobo lokusabela kwe-Catalytic:

Mayelana ne-Amine Dehydrogenase2

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ulwazi Lomkhiqizo:

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-coenzyme kanye nohlelo lokuvuselela i-coenzyme (isb. i-glucose kanye ne-glucose dehydrogenase).
➢ I-AmDH kufanele ifakwe ekugcineni ohlelweni lokusabela, ngemuva kokuthi i-pH kanye nokushisa sekulungisiwe esimweni sokusabela.

Izibonelo Zokusetshenziswa:

Isibonelo 1 (Ukwenziwa kwama-chiral amine ahambisanayo ngokunciphisa i-alkyl aryl ketones)⁽¹⁾:

Isitoreji:

Gcina iminyaka emibili ngaphansi kuka -20℃.

Ukunakwa:

Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.