I-oxidase yotshwala (AOX)
Incazelo emfushane:
Mayelana ne-oxidase yotshwala
Ama-ES-AOX: Lawa ma-enzyme ayakwazi ukuvuselela ukungcoliswa kwama-alcohol anamafutha noma ama-aryl-alcohol ukuze akhe ama-aldehydes. Asebenzisa i-oxygen yama-molecule kodwa awadingi i-cofactor eyengeziwe yangaphandle, kanye ne-hydrogen peroxide ekhiqizwayo.
Kunezinhlobo eziyisithupha zemikhiqizo ye-alcohol oxidases (Inombolo njenge-ES-AOX-101~ES-AOX-106) eyathuthukiswa yi-SyncoZymes. I-ES-AOX101 kanye ne-ES-AOX102 zikhetha ama-substrate e-alcohol aliphatic, i-ES-AOX103~ ES-AOX105 zikhetha ama-substrate e-alcohol anuka kamnandi, kanti i-ES-AOX106 iyi-cholesterol oxidase. I-SZ-AOX iyithuluzi eliwusizo lokuvuselela ukushiswa kwama-alcohol anamafutha noma ama-aryl-alcohols ukukhiqiza ama-aldehydes.
Uhlobo lokusabela kwe-Catalytic:
Iselula/I-Wechat/I-WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | I-ES-AOX-101~ ES-AOX-105 | isethi yama-oxidases ama-5 otshwala, ama-50 mg ngalinye Izinto ezi-5 * 50mg / into, noma olunye ubuningi |
| Ikhithi Yokuhlola (i-SynKit) | I-ES-AOX-500 | isethi yama-oxidases ama-5 otshwala, ama-50 mg ngalinye Izinto ezi-5 * 50mg / into, noma olunye ubuningi |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer kanye ne-ES-AOX, futhi kufanele kube khona umoya-mpilo.
➢ Zonke izinhlobo ze-ES-AOXs ezihambisana nezimo ezahlukahlukene zokusabela ezingcono kakhulu, ezingafundwa ngazinye.
➢ I-substrate ephezulu noma umkhiqizo one-concentration ungavimbela umsebenzi we-ES-AOX. Kodwa-ke, ukuvinjelwa kungancishiswa ngokufaka i-substrate ngenqwaba.
➢ Ukuqongelela kwe-H2O2ohlelweni kuzoholela ekungasebenzini kwama-enzyme, okungaxazululwa ngempumelelo ngokusebenzisa i-catalase.
Isibonelo 1 (Ukushiswa kwe-aryl-alcohols)(1):
Isibonelo 2 (Ukushiswa kwe-oxidation yama-alcohol anamafutha)(2):
Gcina iminyaka emibili ngaphansi kuka -20℃.
Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
1. Benen J AE, Sa'nchez-Torres P, Wagemaker M JM, e tal. J Biol Chem, 1998, 273(14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal. I-Appl Microbiol Biot, 1992, 37: 66-73.
