I-Phenylalanine ammonia lyase (PAL)
Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
I-Enzyme powder | ES-PAL-101~ ES-PAL-110 | isethi ye-10 L-phenylalanine ammonia-lyase, 50 mg into ngayinye 10 * 50mg / into, noma elinye inani |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isisombululo se-buffer kanye ne-enzyme.
➢ Uhlobo ngalunye lwe-ES-PAL oluhambisana nezimo ezihlukahlukene zokusabela, ezingafundwa ngazodwana.
➢ I-ES-PAL kufanele yengezwe okokugcina ohlelweni lokusabela ukuze kugcinwe umsebenzi.
Isibonelo 1(I-Biosynthesis ye-trans cinnamic acid evela ku-phenylalanine)(1):
Isibonelo 2(I-Biosynthesis ye-phenylalanine derivative from trans-cinnamic acid derivative)(2):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. Zachary Mays, Karishma Mohan,et al.I-Chem Commun (Camb).2020, 56, 5255-5258.
2. U-Ian Rowles, u-Bas Groenendaal,et al. I-Tetrahedron,2016. 72, 7343-7347.