I-Nitrile hydratase (NHT)
Incazelo emfushane:
Mayelana ne-ES-NHTs
Ama-ES-NHT: uhlobo lwe-metalloenzyme, olukhetha ukumunca ama-nitrile abe yi-primary amide ehambisanayo. Asetshenziswa kabanzi hhayi kuphela ekwakhiweni kwama-amino acid, ama-amides, ama-carboxylic acid kanye ne-derivatives yawo, kodwa futhi nasekulungiseleleni izidakamizwa ze-chiral. Kunezinhlobo ezingu-24 zama-nitrile hydratases athuthukiswe yi-SyncoZymes (Inombolo njenge-ES-NHT-101～ES-NHT-124).
Uhlobo lokusabela kwe-Catalytic:
Iselula/I-Wechat/I-WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | I-ES-NHT-101~ ES-NHT-124 | isethi yama-Nitrile Hydratases angu-24, 50 mg ngayinye, izinto ezingu-24 * 50mg/into, noma olunye ubuningi |
| Ikhithi Yokuhlola (i-SynKit) | I-ES-NHT-2400 | isethi yama-Nnitrile Hydratases angu-24, 1 mg ngayinye Izinto ezingu-24 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer (i-pH yokusabela efanele) kanye ne-ES-NHT.
➢ Wonke ama-ES-NHT angahlolwa ngokulandelana ohlelweni lokusabela olungenhla noma nge-NHT Screening Kit (SynKit NHT).
➢ Zonke izinhlobo ze-ES-NHTs ezihambisana nezimo ezahlukahlukene zokusabela ezifanele kufanele zifundwe ngazinye.
➢ I-substrate ephezulu noma umkhiqizo one-concentration ungavimbela umsebenzi we-ES-NHT. Kodwa-ke, ukuvinjelwa kungancishiswa ngokungezwa kwe-substrate, ukuhlanganiswa nama-amidase noma ukukhishwa komkhiqizo.
Isibonelo 1 (ukuhlanganiswa ne-amidase)(1):
Isibonelo 2(2):
Gcina iminyaka emibili ngaphansi kuka -20℃.
Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
1 Vojtech V, Ludmila M, Alicja BV, e tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, etal. I-Tetrahedron lett, 2010, 51: 1639-1641.
