I-Nitrile hydratase (NHT)
Incazelo emfushane:
Mayelana ne-ES-NHTs
I-ES-NHTs: isigaba se-metalloenzyme, ekhetha ngokukhetha i-nitriles ku-amide eyinhloko ehambisanayo.Asetshenziswa kabanzi hhayi kuphela ukuhlanganiswa kwama-amino acid, ama-amide, ama-carboxylic acid kanye nokuphuma kwawo, kodwa futhi nasekulungiseleleni izidakamizwa ze-chiral.Kunezinhlobo ezingama-24 zama-nitrile hydratases athuthukiswe yi-SyncoZymes (Inombolo njenge-ES-NHT-101～ES-NHT-124).
Uhlobo lokusabela kwe-Catalytic:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
| I-Enzyme Powder | ES-NHT-101~ ES-NHT-124 | isethi 24 Nitrile Hydratase, 50 mg ngayinye 24 izinto * 50mg / into, noma enye ubuningi |
| Ikhithi Yokuhlola (SynKit) | ES-NHT-2400 | isethi 24 Nnitrile Hydratase, 1 mg ngayinye 24 izinto * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo sebhafa (i-pH yokusabela ekahle) kanye ne-ES-NHT.
➢ Wonke ama-ES-NHT angahlolwa ngokulandelana kwawo ohlelweni lokusabela olungenhla noma nge-NHT Screening Kit (SynKit NHT).
➢ Zonke izinhlobo ze-ES-NHT ezihambisana nezimo ezihlukile zokusabela ezifanele kufanele zifundwe ngazodwana.
➢ I-Substrate egxilile ephezulu noma umkhiqizo onawo ungase uvimbele umsebenzi we-ES-NHT.Kodwa-ke, ukuvimbela kungakhululwa ngokungezwa kwe-batch ye-substrate, kuhlanganiswe nama-amidases noma ukukhishwa komkhiqizo.
Isibonelo 1 (ukuhlanganisa ne-amidase)(1):
Isibonelo sesi-2(2):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1 Vojtech V, Ludmila M, Alicja BV, e tal.J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, e tal.I-Tetrahedron lett, 2010, 51: 1639-1641.
