Isithombe Esifakiwe se-Ketoreductase (KRED)

I-Ketoreductase (KRED)

Incazelo emfushane:

Mayelana ne-Ketoreductase

Ama-enzyme: Angama-catalyst ebhayoloji e-macromolecular, ama-enzyme amaningi angamaprotheni.

I-Ketoreductase: Ebizwa nangokuthi “i-Carbonyl reductase”, ama-enzyme avuselela ukusabela kwe-redox okuguqukayo phakathi kwama-aldehydes/ketones nama-alcohols, futhi adinga ama-coenzymes njenge-H transmitter njenge-NADH noma i-NADPH. Kunezinhlobo ezintathu eziyinhloko ze-KRED: Ama-Short-chain Dehydrogenase / Reductases (ama-SDR), ama-Medium-chain Dehydrogenases / Reductases (ama-MDR), kanye nama-Aldo-KetoReductases (ama-AKR). Imisebenzi yokuvuselela yalezi zintathu iyafana, kodwa isakhiwo kanye nezakhiwo zihlukile kakhulu.

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Mayelana ne-Ketoreductase:

I-KRED evela ku-SyncoZymes: Kunezinhlobo ezingu-191 zemikhiqizo ye-enzyme ye-KRED (Inombolo njenge-ES-KRED-101~ES-KRED-291) eyenziwe yi-SyncoZymes. I-SZ-KRED ingasetshenziswa kabanzi ekunciphiseni ama-aldehydes, ama-ester e-beta-ketone, ama-ester e-α-ketone nama-ketone ngokwesifunda kanye nangokwe-stereoselective.

Uhlobo lokusabela kwe-Catalytic:

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Imininingwane
Impuphu ye-Enzyme	Impuphu ye-Enzyme	isethi yama-Ketoreductase angu-187, angu-50 mg ngalinye Izinto ezingu-187 * 50mg / into, noma olunye ubuningi
Ikhithi Yokuhlola (i-SynKit)	I-ES-KRED-18100	isethi yama-Ketoreductase angu-181, 1 mg ngayinye Izinto ezingu-181 * 1mg / into

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukusebenza kahle kokuguqulwa.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ukuhlolwa kwama-enzyme kufanele kwenziwe kuma-substrate athile ngenxa yobukhulu be-substrate, bese kutholwa i-enzyme evuselela i-substrate eqondiwe ngomphumela omuhle kakhulu we-catalytic.
➢ Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, ama-coenzyme (i-NAD(H) noma i-NADP(H)), uhlelo lokuvuselela ama-coenzyme (isib. i-glucose kanye ne-glucose dehydrogenase).
➢ I-KRED kufanele ifakwe ekugcineni ohlelweni lokusabela, ngemuva kokuthi i-pH kanye nokushisa kulungiswe esimweni sokusabela.
➢ Zonke izinhlobo ze-KRED zinezimo ezahlukene zokusabela ezingcono kakhulu, ngakho-ke ngayinye yazo kufanele ifundwe kabanzi ngayinye.