Isithombe Esifakiwe Se-CALB Esingenakunyakaziswa

I-CALB engakwazi ukuhamba

Incazelo emfushane:

I-CALB

I-Recombinant Lipase B evela ku-Candida antarctica (CALB) ikhiqizwa ngokuvutshelwa okungaphansi kwamanzi nge-Pichia pastoris eguqulwe ngofuzo.

I-CALB ingasetshenziswa esigabeni samanzi noma isigaba se-organic catalytic esterification, i-esterolysis, i-transesterification, i-polyester yokuvula indandatho, i-aminolysis, i-hydrolysis yama-amides, i-acylation yama-amine kanye nokusabela kokwengeza.

I-CALB inokukhetha okuphezulu kwe-chiral kanye nokukhetha isikhundla, ngakho-ke ingasetshenziswa kabanzi ekucubunguleni uwoyela, ukudla, imithi, izimonyo nezinye izimboni zamakhemikhali.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

I-CALB engakwazi ukuhamba:

I-CALB ayinyakazi ngenxa yokukhangisa ngokomzimba ku-resin ene-hydrophobic ephezulu eyi-macroporous, styrene/methacrylate polymer.I-Immobilized CALB ilungele ukusetshenziswa kuzincibilikisi zemvelo kanye nezinhlelo ezingenazo izincibilikisi, futhi ingagaywa kabusha futhi isetshenziswe kabusha izikhathi eziningi ezimeni ezifanele.
Ikhodi Yomkhiqizo: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Izinzuzo ze-SZ-CALB-IMMO100:

★Umsebenzi ophezulu, ukukhetha okuphezulu kwe-chiral nokuzinza okuphezulu.
★Ukusebenza okungcono ezigabeni ezingenamanzi.
★Susa kalula ohlelweni lokusabela, nqamula ukusabela ngokushesha, futhi ugweme insalela yamaprotheni emkhiqizweni.
★Ingasetshenziswa kabusha futhi isetshenziswe kabusha ukuze kwehliswe izindleko.

Amapharamitha womkhiqizo we-SZ-CALB- IMMO100:

Umsebenzi	≥10000PLU/g
Ibanga le-pH lokusabela	5-9
Ibanga lokushisa lokusabela	10-60 ℃
Ukubukeka	I-CALB-IMMO100-A: Okuphuzi okukhanyayo kuya kokuqinile okunsundu I-CALB-IMMO100-B: Okuqinile okumhlophe kuya kokunsundu ngokukhanyayo
Usayizi wezinhlayiya	300-500μm
Ukulahlekelwa ekumisweni ku-105 ℃	0.5%-3.0%
I-resin ye-immobilization	I-Macroporous, i-styrene/methacrylate polymer
I-solvent yokusabela	Amanzi, i-organic solvent, njll., noma ngaphandle kwe-solvent.Ngokusabela kwezinye izinyibilikisi eziphilayo, amanzi angu-3% anganezelwa ukuthuthukisa umphumela wokusabela
Usayizi wezinhlayiya	I-CALB-IMMO100-A: 200-800 μm I-CALB-IMMO100-B: 400-1200 μm

Incazelo yeyunithi: Iyunithi engu-1 ihambisana nokuhlanganiswa kwe-1μmol ngomzuzu we-propyl laurate kusuka ku-lauric acid ne-1-propanol ku-60℃.I-CALB-IMMP100-A engenhla ne-CALB-IMMO100-B ihambisana nabathwali abangakwazi ukuhamba abanosayizi abahlukene bezinhlayiyana.

Imiyalelo nezinyathelo zokuqapha:

1. Uhlobo lwe-reactor
I-enzyme enganyakazi iyasebenza kukho kokubili i-kettle batch reactor kanye ne-fixed bed continuous flow reactor.Kumele kuqashelwe ukugwema ukuchotshozwa ngenxa yamandla angaphandle ngesikhathi sokudla noma ukugcwalisa.
2. I-pH yokusabela, izinga lokushisa kanye ne-solvent
I-enzyme enganyakazi kufanele yengezwe ekugcineni, ngemuva kokuthi ezinye izinto zengezwe futhi zihlakazwe, futhi i-pH ilungiswe.
Uma ukusetshenziswa kwe-substrate noma ukwakhiwa komkhiqizo kuzoholela ekushintsheni kwe-pH ngesikhathi sokusabela, isigcinalwazi esanele kufanele sengezwe ohlelweni lokusabela, noma i-pH kufanele iqashwe futhi ilungiswe ngesikhathi sokusabela.
Ngaphakathi kwebanga lokubekezelela izinga lokushisa le-CALB (ngaphansi kuka-60 ℃), izinga lokuguqulwa liyenyuka ngokunyuka kwezinga lokushisa.Ekusetshenzisweni okungokoqobo, izinga lokushisa lokusabela kufanele likhethwe ngokuvumelana nokuzinza kwe-substrate noma umkhiqizo.
Ngokuvamile, ukusabela kwe-ester hydrolysis kufanelekile ohlelweni lwesigaba esinamanzi, kuyilapho ukusabela kwe-ester synthesis kufanelekile ohlelweni lwesigaba sezinto eziphilayo.I-solvent ephilayo ingaba i-ethanol, i-tetrahydrofuran, i-n-hexane, i-n-heptane ne-toluene, noma i-solvent ehlanganisiwe efanelekile.Ngokusabela kwezinye izinyibilikisi eziphilayo, amanzi angu-3% anganezelwa ukuthuthukisa umphumela wokusabela.
3. Ukusetshenziswa kabusha nempilo yesevisi ye-CALB
Ngaphansi kwesimo sokusabela esifanele, i-CALB ingatholwa futhi iphinde isetshenziswe, futhi izikhathi zohlelo lokusebenza ziyahlukahluka ngamaphrojekthi ahlukene.
Uma i-CALB etholiwe ingaphinde isetshenziswe ngokuqhubekayo futhi idinga ukugcinwa ngemva kokululama, idinga ukuwashwa futhi yomiswe futhi ivalwe ku-2-8 ℃.
Ngemuva kwemizuliswano embalwa yokusetshenziswa kabusha, uma ukusebenza kahle kokusabela kwehliswa kancane, i-CALB ingangezwa ngokufanelekile futhi iqhubeke nokusebenzisa.Uma ukusebenza kahle kokusabela kuncipha kakhulu, kudinga ukushintshwa.

Izibonelo Zohlelo Lokusebenza:

Isibonelo 1(Aminolysis)⁽¹⁾:

Isibonelo 2(Aminolysis)⁽²⁾:

Isibonelo 3(I-polyester yokuvula indandatho)⁽³⁾:

Isibonelo 4(I-Transesterification, i-regioselective yeqembu le-hydroxyl)⁽⁴⁾:

Isibonelo sesi-5(Transesterification, ukulungiswa kwe-kinetic kotshwala be-racemic)⁽⁵⁾:

Isibonelo 6(I-Esterification, ukulungiswa kwe-kinetic ye-carboxylic acid)⁽⁶⁾:

Isibonelo 7(I-Esterolysis, ukulungiswa kwe-kinetic)⁽⁷⁾:

Isibonelo 8(I-Hydrolysis of amides)⁽⁸⁾:

Isibonelo 9(Acylation of amines)⁽⁹⁾:

Isibonelo 10(Aza-Michael add reaction)⁽¹⁰⁾:

Izithenjwa:

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