I-CALB Engashukumi

Incazelo emfushane:

I-CALB

I-Recombinant Lipase B evela ku-Candida antarctica (CALB) ikhiqizwa ngokubiliswa okucwiliswe emanzini nge-Pichia pastoris eguquliwe ngokwezakhi zofuzo.

I-CALB ingasetshenziswa esigabeni samanzi noma esigabeni se-organic catalytic esterification, i-esterosis, i-transesterification, i-ring opening polyester synthesis, i-aminolysis, i-hydrolysis yama-amides, i-acylation yama-amine kanye nokusabela kokwengeza.

I-CALB inokukhetha okuphezulu kwe-chiral kanye nokukhetha kwesikhundla, ngakho-ke ingasetshenziswa kabanzi ekucubungulweni kwamafutha, ekudleni, emithini, ezimonyweni nakwezinye izimboni zamakhemikhali.

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

I-CALB Engashukumi:

I-CALB ayishukunyiswa ukumuncwa ngokomzimba ku-resin emanzi kakhulu eyi-polymer enezimbobo ezinkulu, i-styrene/methacrylate. I-Immobilised CALB ifanele ukusetshenziswa ezincibilikisini eziphilayo kanye nezinhlelo ezingenazo izincibilikisi, futhi ingaphinde isetshenziswe futhi isikhathi esiningi ezimweni ezifanele.
Ikhodi Yomkhiqizo: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Izinzuzo ze-SZ-CALB-IMMO100:

★Umsebenzi ophakeme, ukukhetha okuphezulu kwe-chiral kanye nokuzinza okuphezulu.
★Ukusebenza okungcono ezigabeni ezingezona ezamanzi.
★Khipha kalula ohlelweni lokusabela, uqede ngokushesha ukusabela, futhi ugweme izinsalela zamaprotheni kumkhiqizo.
★Ingaphinde isetshenziswe futhi isetshenziswe kabusha ukuze kuncishiswe izindleko.

Amapharamitha Omkhiqizo we-SZ-CALB-IMMO100:

Umsebenzi	≥10000PLU/g
ububanzi be-pH bokusabela	5-9
Ububanzi bokushisa bokusabela	10-60℃
Ukubukeka	I-CALB-IMMO100-A: Okuqinile okuphuzi okukhanyayo kuya kokunsundu I-CALB-IMMO100-B: Okumhlophe kuya kokunsundu okukhanyayo okuqinile
Usayizi wezinhlayiya	300-500μm
Ukulahlekelwa lapho komiswa ku-105℃	0.5%-3.0%
I-resin yokuvimbela ukuhamba	I-polymer enezimbobo ezinkulu, i-styrene/methacrylate
Isinyibilikisi sokusabela	Amanzi, i-organic solvent, njll., noma ngaphandle kwe-solvent. Ukuze uthole ukusabela kwezinye izinyibilikisi ze-organic, amanzi angu-3% angangezwa ukuthuthukisa umphumela wokusabela
Usayizi wezinhlayiya	I-CALB-IMMO100-A: 200-800 μm I-CALB-IMMO100-B: 400-1200 μm

Incazelo yeyunithi: Iyunithi eli-1 lihambisana nokuhlanganiswa kwe-1μmol ngomzuzu we-propyl laurate kusuka ku-lauric acid kanye ne-1-propanol ku-60℃. I-CALB-IMMP100-A kanye ne-CALB-IMMO100-B engenhla zihambisana nabathwali abangakwazi ukuhamba abanobukhulu obuhlukene bezinhlayiya.

Imiyalelo kanye Nezinyathelo Zokuqapha:

1. Uhlobo lwe-reactor
I-enzyme engashukumi iyasebenza kokubili ku-kettle batch reactor kanye ne-fixed bed continuous flow reactor. Kufanele kuqashelwe ukuthi kugwenywe ukuchotshozwa ngenxa yamandla angaphandle ngesikhathi sokudla noma ukugcwalisa.
2. I-pH yokusabela, izinga lokushisa kanye nesinyibilikisi
I-enzyme engasebenzi kahle kufanele ifakwe ekugcineni, ngemva kokungezwa kwezinye izinto nokuncibilikiswa, bese i-pH ilungiswa.
Uma ukusetshenziswa kwe-substrate noma ukwakheka komkhiqizo kuzoholela ekushintsheni kwe-pH ngesikhathi sokusabela, kufanele kwengezwe i-buffer eyanele ohlelweni lokusabela, noma i-pH kufanele iqashwe futhi ilungiswe ngesikhathi sokusabela.
Ngaphakathi kwebanga lokubekezelela izinga lokushisa le-CALB (ngaphansi kuka-60 ℃), izinga lokuguqulwa landa ngokukhuphuka kwezinga lokushisa. Ekusetshenzisweni okungokoqobo, izinga lokushisa lokusabela kufanele likhethwe ngokuya ngokuzinza kwe-substrate noma umkhiqizo.
Ngokuvamile, ukusabela kwe-ester hydrolysis kufanelekile ohlelweni lwesigaba samanzi, kuyilapho ukusabela kokwenziwa kwe-ester kufanelekile ohlelweni lwesigaba sezinto eziphilayo. I-solvent yezinto eziphilayo ingaba i-ethanol, i-tetrahydrofuran, i-n-hexane, i-n-heptane kanye ne-toluene, noma i-solvent exubile efanele. Ukuze kutholakale ukusabela kwezinye izinyibilikisi zezinto eziphilayo, amanzi angu-3% angangezwa ukuthuthukisa umphumela wokusabela.
3. Ukusetshenziswa kabusha kanye nesikhathi senkonzo ye-CALB
Ngaphansi kwesimo esifanele sokusabela, i-CALB ingatholwa futhi isetshenziswe kabusha, futhi izikhathi ezithile zokufaka ziyahlukahluka ngamaphrojekthi ahlukene.
Uma i-CALB etholiwe ingasetshenziswa njalo futhi idinga ukugcinwa ngemva kokubuyiselwa, idinga ukugezwa, yomiswe bese ivalwa ku-2-8 ℃.
Ngemva kokusetshenziswa kabusha izikhathi eziningana, uma ukusebenza kahle kokusabela kuncishisiwe kancane, i-CALB ingangezwa ngendlela efanele futhi iqhubeke nokusetshenziswa. Uma ukusebenza kahle kokusabela kuncishisiwe kakhulu, idinga ukushintshwa.

Izibonelo Zokusetshenziswa:

Isibonelo 1 (i-Aminolysis)⁽¹⁾:

Isibonelo 2 (i-Aminolysis)⁽²⁾:

Isibonelo 3 (Ukwenziwa kwe-polyester evula iringi)⁽³⁾:

Isibonelo 4 (Ukuguqulwa kwe-esterification, ukukhetha kabusha kweqembu le-hydroxyl)⁽⁴⁾:

Isibonelo 5 (Ukuguqulwa kwe-ester, ukuxazululwa kwe-kinetic kwe-racemic alcohols)⁽⁵⁾:

Isibonelo 6 (Ukuqinisekiswa, ukulungiswa kwe-kinetic kwe-carboxylic acid)⁽⁶⁾:

Isibonelo 7 (i-Esterolysis, i-kinetic resolution)⁽⁷⁾:

Isibonelo 8 (i-Hydrolysis yama-amides)⁽⁸⁾:

Isibonelo 9 (Ukwakheka kwama-amine)⁽⁹⁾:

Isibonelo 10 (ukusabela kokungezwa kuka-Aza-Michael)⁽¹⁰⁾:

Izinkomba:

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7. Souza TC, Fonseca TS, Costa JA, e tal. J. Mol. I-Catal. B: I-Enzym, 2016, 130: 58-69.
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9. Joubioux FL, Henda YB, Bridiau N, e tal. J. Mol. I-Catal. B: I-Enzym, 2013, 85-86: 193-199.
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