IHydroaminase (HAM)
Incazelo emfushane:
Mayelana neHydroaminase
Kunezinhlobo ezi-2 zomkhiqizo we-enzyme ye-HAM (Inombolo njengokuthi ES-HAM-101~ ES-HAM-102) eyakhiwe yi-SyncoZymes.I-HAM ingakwazi ukuvuselela i-ammoniation ye-enoic acid noma okuphuma kuyo ukukhiqiza ama-chiral amino acid.I-HAM ingasetshenziswa ukwenza ama-chiral amino acid (noma ama-chiral amines) asuka kuma-enoic acid (noma ama-alkenes).Umnikezeli we-ammonia, njengamanzi ammonia noma usawoti we-ammonium, uyadingeka.
Uhlobo lokusabela lwe-Hydroaminase
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
| I-Enzyme Powder | ES-HAM-101~ ES-HAM-102 | isethi ye-2 Nuclosidephosphoryalse, 50 mg ngayinye izinto ezi-2 * 50mg / into, noma elinye inani |
Isistimu yokusabela elandelayo ingasetshenziswa njengereferensi:
1-10 mg/ml: I-Substrate
10 mg/ml: I-enzyme
10% (v/v): I-Organic cosolvent (1)
10% (v/v): I-Amoniya
Lungisa i-pH ibe ngu-9.0 ngaphambi kokwengeza i-enzyme, futhi i-enzyme kufanele yengezwe ekugcineni.Fukamisa ukusabela endaweni enyakazisa izinga lokushisa engashintshi (isibonelo, 30oC, 150rpm) amahora angama-24~48.I-Centrifuge ingxube ngayinye ukuze iphuze amaprotheni, wona angaphezu kwamandla angasetshenziselwa ukuhlaziya i-TLC, HPLC noma i-GC.
Qaphela (1): Isinyibilikisi se-Organic (njenge-DMSO, i-methanol, i-acetonitrile, i-IPA, njll.) iyakhethwa, isetshenziselwa ama-substrates angancibiliki.
