I-Hydroaminase (HAM)
Incazelo emfushane:
Mayelana ne-Hydroaminase
Kunezinhlobo ezimbili zomkhiqizo we-enzyme ye-HAM (Inombolo njenge-ES-HAM-101~ ES-HAM-102) eyathuthukiswa yi-SyncoZymes. I-HAM ingavuselela i-ammonia ye-enoic acid noma izinto eziphuma kuyo ukuze ikhiqize ama-amino acid e-chiral. I-HAM ingasetshenziswa ukuhlanganisa ama-amino acid e-chiral (noma ama-chiral amines) avela kuma-enoic acid (noma ama-alkenes). Umnikeli we-ammonia, njengamanzi e-ammonia noma usawoti we-ammonium, uyadingeka.
Uhlobo lokusabela kwe-Hydroaminase catalytic
Iselula/I-Wechat/I-WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | I-ES-HAM-101~ I-ES-HAM-102 | isethi yama-Nuclosidephosphoryalse ama-2, ama-50 mg ngalinye izinto ezi-2 * 50mg / into, noma olunye ubuningi |
Uhlelo lokusabela olulandelayo lungasetshenziswa njengereferensi:
1-10 mg/ml: I-Substrate
10 mg/ml: I-enzyme
10% (v/v): I-cosolvent yemvelo (1)
10% (v/v): i-Amoniya
Lungisa i-pH ibe ngu-9.0 ngaphambi kokufaka i-enzyme, bese i-enzyme kufanele ingezwe ekugcineni. Faka ukusabela ku-temperature shaker engaguquki (isibonelo, 30oC, 150rpm) amahora angama-24 ~ 48. Faka i-centrifuge ingxube ngayinye ukuze i-protein isheshe, lapho i-supernatant ingasetshenziswa khona ekuhlaziyeni kwe-TLC, HPLC noma i-GC.
Qaphela (1): Isinyibilikisi se-organic (njenge-DMSO, i-methanol, i-acetonitrile, i-IPA, njll.) singakhethwa, sisetshenziselwa izinto ezinganyibiliki.
