I-Epoxide hydrolase (EH)
Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
I-Enzyme powder | ES-EH-101~ ES-EH-103 | isethi 3 Epoxide Hydrolases, 50 mg ngayinye 3 izinto * 50mg / into, noma omunye ubuningi |
Ikhithi Yokuhlola (SynKit) | ES-EH-300 | isethi 3 Epoxide Hydrolases, 1 mg ngayinye 3 izinto * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo se-buffer (i-pH yokusabela efanelekile) kanye ne-ES-EH.
➢ Zonke izinhlobo ze-ES-EHs ezihambisana nezimo zokusabela ezifanele kufanele zifundwe ngazodwana.
➢ I-Substrate egxilile ephezulu noma umkhiqizo onawo ungase uvimbele umsebenzi we-ES-EH.Kodwa-ke, ukuvinjelwa kungakhululeka ngokungezwa kwe-batch ye-substrate.
Isibonelo 1(1):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. Kim YH, Lee I, Choi SH, e tal.J Mol Catal B-Enzym, 2013, 89: 48-51.