SyncoZymes

imikhiqizo

I-Epoxide hydrolase (EH)

Incazelo emfushane:

Mayelana ne-epoxide hydrolase

I-EH i-ether hydrolase eyenza i-stereoelective hydrolysis yama-racemic epoxides ukukhiqiza i-1, 2-diol kanye nama-epoxide asebenzayo, angasetshenziselwa ukulungiswa kwe-chiral yama-epoxides noma ukulungiswa kwama-chiral diols.Asetshenziswa kakhulu kwezokwelapha nakweminye imikhakha.Kunezinhlobo ezi-3 zemikhiqizo ye-epoxide hydrolases (Inombolo njengokuthi ES-EH-101~ES-EH-103) ethuthukiswe yi-SyncoZymes.I-ES-EH iyithuluzi eliwusizo lokugqugquzela ukuhlanganiswa kotshwala be-chiral ngokusebenzisa i-stereoelective hydrolysis yama-aliphatic and aromatics epoxides.

Uhlobo lokusabela kwe-Catalytic:

I-Epoxide hydrolase EH2

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ulwazi Lomkhiqizo:

I-Epoxide hydrolase EH
Ama-enzyme Ikhodi Yomkhiqizo Ukucaciswa
I-Enzyme powder ES-EH-101~ ES-EH-103 isethi 3 Epoxide Hydrolases, 50 mg ngayinye 3 izinto * 50mg / into, noma omunye ubuningi
Ikhithi Yokuhlola (SynKit) ES-EH-300 isethi 3 Epoxide Hydrolases, 1 mg ngayinye 3 izinto * 1mg / into

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.

Imiyalo yokusebenzisa:

➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo se-buffer (i-pH yokusabela efanelekile) kanye ne-ES-EH.
➢ Zonke izinhlobo ze-ES-EHs ezihambisana nezimo zokusabela ezifanele kufanele zifundwe ngazodwana.
➢ I-Substrate egxilile ephezulu noma umkhiqizo onawo ungase uvimbele umsebenzi we-ES-EH.Kodwa-ke, ukuvinjelwa kungakhululeka ngokungezwa kwe-batch ye-substrate.

Izibonelo Zohlelo Lokusebenza:

Isibonelo 1(1):

I-Epoxide hydrolase EH3

Isitoreji:

Gcina iminyaka emi-2 ngaphansi -20 ℃.

Qaphela:

Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.

Izithenjwa:

1. Kim YH, Lee I, Choi SH, e tal.J Mol Catal B-Enzym, 2013, 89: 48-51.


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