I-Benzaldehyde lyase (BAL)
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isisombululo se-buffer, i-enzyme, i-ThDP, i-Mg2+.
➢ I-BAL kufanele yengezwe okokugcina ohlelweni lokusabela, ngemuva kokuthi i-pH nezinga lokushisa kulungiswe esimweni sokusabela.
Isibonelo 1(Ukuhlanganiswa kwe-N-efakwe esikhundleni se-1,2 amino alcohols evela ku-aldehydes)(1):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. U-Li Y, u-Hu N, u-Xu Z, nabanye.I-Angewandte Chemie International Edition, 2022, 61(17): e202116344.