Isithombe Esifakiwe se-Phosphokinase (PKase)

I-Phosphokinase (PKase)

Incazelo emfushane:

Mayelana ne-Phosphokinase

I-ES-PKase (i-Phosphokinase): ivuselela ukudluliselwa kwamaqembu e-phosphate ku-ATP kwamanye ama-compounds. Iphinde ivuselele ukudluliselwa kwamaqembu e-phosphate kwamanye ama-nucleoside e-triphosphate ngezinye izikhathi. Ama-kinase amaningi adinga ama-ion ensimbi ahlukene ukuze ahlanganyele ekusabeleni (ngokuvamile i-Mg2+). Kunezinhlobo ezingu-21 zemikhiqizo ye-enzyme ye-PKase (Inombolo njenge-ES-PKase101~ ES-PKase-121) eyenziwe yi-SyncoZymes.
Uhlobo lokusabela kwe-Catalytic:

I-Phosphokinase PKase2

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Imininingwane
Ikhithi Yokuhlola (i-SynKit)	I-ES-PKase-101~ES-PKase-121	isethi yama-Ketoreductase angu-21, 1 mg ngayinye Izinto ezingu-21 * 1mg / into

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-ATP, i-Mg²⁺.
➢ I-PKase kufanele ifakwe ekugcineni ohlelweni lokusabela, ngemuva kokuthi i-pH kanye nokushisa kulungiswe esimweni sokusabela.

Izibonelo Zokusetshenziswa:

Isibonelo 1 (Ukwenziwa kwe-nicotinamide ribose phosphate kusuka ku-nicotinamide ribose)⁽¹⁾:

Qaphela: izibonelo zohlelo lokusebenza kanye nezinkomba zenzelwe ukukhombisa ububanzi bokusetshenziswa kwe-PKase ukuze kube lula ukuqonda, futhi azihambisani ne-enzyme ethile ye-SyncoZymes.

Isitoreji:

Gcina iminyaka emibili ngaphansi kuka -20℃.

Ukunakwa:

Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.