I-Phosphokinase (PKase)
Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
Ikhithi Yokuhlola (SynKit) | ES-PKase-101~ES-PKase-121 | isethi 21 Ketoreductases, 1 mg ngayinye 21 izinto * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isisombululo se-buffer, i-enzyme, i-ATP, i-Mg2+.
➢ I-PCase kufanele yengezwe okokugcina ohlelweni lokusabela, ngemva kokuthi i-pH nezinga lokushisa kulungiswe esimweni sokusabela.
Isibonelo 1(Ukwenziwa kwe-nicotinamide ribose phosphate kusuka ku-nicotinamide ribose)(1):
Qaphela: izibonelo zohlelo lokusebenza nezithenjwa zihloselwe ukukhombisa ububanzi bohlelo lwe-PCase ukuze kube lula ukuqonda, futhi azihambisani ne-enzyme ethile ye-SyncoZymes.
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. Khan, Javed A., Song Xiang, and Liang Tong.Isakhiwo 15.8 (2007): 1005-1013.