Isithombe Esifakiwe se-Nucloside phosphorylse (NP)

I-Nucloside phosphorylse (NP)

Incazelo emfushane:

Mayelana ne-nucleoside phosphorylse

Kunezinhlobo ezintathu zemikhiqizo ye-enzyme ye-NP (Inombolo njenge-ES-NP-101~ ES-NP-103) eyathuthukiswa yi-SyncoZymes. I-ES-NP-101 yi-purine nucleoside phosphorylase, i-ES-NP-102 kanye ne-ES-NP-103 yi-pyrimidine nucleoside phosphorylase. I-Nucleosidephosphorylase ingahlukanisa ama-nucleoside abe yizisekelo kanye ne-pentose phosphate. I-Nucleoside phosphorylase ingahlukaniswa ibe yi-purine nucleoside phosphorylase kanye ne-pyrimidine nucleoside phosphorylase ngokuya ngokuthanda kwezisekelo ze-nucleoside. I-purine nucleoside phosphorylase ingaguqula i-adenosine ibe yi-adenine, i-inosine ibe yi-hypoxanthine, i-guanosine ibe yi-guanine, futhi ikhiqize i-ribose phosphate ngesikhathi esifanayo. I-Pyrimidine nucleoside phosphorylase ingaguqula i-uridine ibe yi-uracil futhi ikhiqize i-ribose phosphate.

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I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Uhlobo lokusabela kwe-Nucloside phosphorylse:

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Imininingwane
Impuphu ye-Enzyme	I-ES-NP-101~ ES-NP-103	isethi yama-Nuclosidephosphoryalse ama-3, ama-50 mg ngalinye izinto ezi-3 * 50mg / into, noma olunye ubuningi

Inqubo yokuhlola ukusabela kwe-Nucloside phosphorylse:

Uhlelo lokusabela olulandelayo lungasetshenziswa njengereferensi:
I-substrate engu-1-10 mg/ml
I-enzyme engu-10 mg/ml
I-100 mM Phosphate buffer (pH7.0)
Faka ukusabela ku-temperature shaker engaguquki (isibonelo, 30oC, 150rpm) amahora ayi-12 ~ 24. Faka i-centrifuge ingxube ngayinye ukuze i-protein isheshe, bese i-supernatant ingasetshenziswa ekuhlaziyeni i-TLC, i-HPLC noma i-GC.