I-Nucloside phosphoryalse (NP)

Incazelo emfushane:

Mayelana ne-nucleoside phosphoryalse

Kunezinhlobo ezi-3 zemikhiqizo ye-enzyme ye-NP (Inombolo njengokuthi ES-NP-101~ ES-NP-103) eyakhiwe yi-SyncoZymes.I-ES-NP-101 i-purine nucleoside phosphorylase, i-ES-NP-102 ne-ES-NP-103 yi-pyrimidine nucleoside phosphorylase.I-Nucleosidephosphorylase ingabola ama-nucleoside abe yizisekelo kanye ne-pentose phosphate.I-Nucleoside phosphorylase ingahlukaniswa ibe yi-purine nucleoside phosphorylase ne-pyrimidine nucleoside phosphorylase ngokuvumelana nokuthandwa kwezisekelo ze-nucleoside.I-Purine nucleoside phosphorylase ingakwazi ukugaya i-adenosine ku-adenine, inosine ku-hypoxanthine, i-guanosine ku-guanine, futhi ikhiqize i-ribose phosphate ngesikhathi esifanayo.I-Pyrimidine nucleoside phosphorylase ingakwazi ukugaya i-uridine ku-uracil futhi ikhiqize i-ribose phosphate.

Iselula/Wechat/WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Uhlobo lokusabela lwe-Nucloside phosphoryalse:

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Ukucaciswa
I-Enzyme Powder	ES-NP-101~ ES-NP-103	isethi ye-3 Nuclosidephosphoryalse, 50 mg ngayinye 3 izinto * 50mg / into, noma elinye inani

Inqubo yokuhlolwa kwe-Nucloside phosphoryalse:

Isistimu yokusabela elandelayo ingasetshenziswa njengereferensi:
I-Substrate engu-1-10 mg/ml
10 mg/ml i-enzyme
100 mM Ibhafa ye-Phosphate (pH7.0)
Fukamisa ukusabela endaweni enyakazisa izinga lokushisa engashintshi (isibonelo, 30oC, 150rpm) amahora angu-12–24.Faka i-Centrifuge ingxube ngayinye ukuze uthole amaprotheni, wona amandla angaphezu kwamandla angasetshenziselwa ukuhlaziywa kwe-TLC, HPLC noma i-GC.