I-Nitrilase (i-NIT)
Incazelo emfushane:
Mayelana ne-Nitrilase
Ama-ES-NIT: Guqula ngqo iqembu le-cyanide libe yiqembu le-carboxyl, ukulungiselela i-carboxylic acid, esetshenziswa kabanzi kwezokwelapha, ekuvikelweni kwemvelo, embonini yamakhemikhali, ekungezeni kokudla, ezindwangu, ekukhiqizweni kwezolimo nakwezinye izinkambu.
Kunezinhlobo ezingu-40 zemikhiqizo ye-Nitrilase (NIT) (Inombolo njenge-ES-NIT-101~ES-NIT-140) eyenziwe yi-SyncoZymes. I-ES-NIT iyithuluzi eliwusizo lokuvuselela ukwenziwa kwama-chiral carboxylic acid ngokusebenzisa i-regio- kanye ne-stereoselective hydrolysis yohlu olubanzi lwama-nitrile e-aliphatic kanye ne-aromatic.
Uhlobo lokusabela kwe-Catalytic:
Iselula/I-Wechat/I-WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | I-ES-NIT-101~ ES-NIT-140 | isethi yama-Nitrilase angu-40, ama-50 mg ngalinye izinto ezingu-40 * 50mg / into, noma olunye ubuningi |
| Ikhithi Yokuhlola (i-SynKit) | I-ES-NIT-4000 | isethi yama-Nitrilase angu-40, 1 mg ngayinye Izinto ezingu-40 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer (i-pH yokusabela efanele) kanye ne-ES-NIT.
➢ Wonke ama-ES-NIT angahlolwa ngokulandelana ohlelweni lokusabela olungenhla noma nge-NIT Screening Kit (SynKit NIT).
➢ Zonke izinhlobo ze-ES-NIT ezihambisana nezimo ezahlukahlukene zokusabela ezifanele kufanele zifundwe ngazinye.
➢ I-substrate eningi noma umkhiqizo one-concentration ephezulu ungavimbela umsebenzi we-ES-NIT. Kodwa-ke, ukuvinjelwa kungancishiswa ngokufaka i-substrate ngenqwaba.
Isibonelo 1 (Ukwenziwa kwe-nicotinic acid)(1):
Isibonelo 2 (Ukwenziwa kwe-(R)-(-)-mandelic acid)(2):
Isibonelo 3 (Ukwenziwa kwe-baclofen)(3):
Isibonelo 4 (Ukwenziwa kwe-glycolic acid)(4):
Gcina iminyaka emibili ngaphansi kuka -20℃.
Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
1 I-CD kaMathew, uNagasawa T, uKobayashi M, etal. I-Appl. Environ. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Uhlelo lokusebenza. Imvelo. I-Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, e tal. Isilevu. J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, no tal. I-Biotechnol. I-Bioeng., 2008, 99(3): 717.
