I-Nitrilase (NIT)
Incazelo emfushane:
Mayelana ne-Nitrilase
I-ES-NITs: Guqula ngokuqondile iqembu le-cyanide libe yiqembu le-carboxyl, ukulungiselela i-carboxylic acid, esetshenziswa kabanzi kwezokwelapha, ukuvikelwa kwemvelo, imboni yamakhemikhali, izithasiselo zokudla, izindwangu, ukukhiqizwa kwezolimo neminye imikhakha.
Kunezinhlobo ezingu-40 zemikhiqizo ye-Nitrilase(NIT) (Inombolo njenge-ES-NIT-101~ES-NIT-140) eyakhiwe ngabakwa-SyncoZymes.I-ES-NIT iyithuluzi eliwusizo lokwenza ukuhlanganiswa kwe-chiral carboxylic acid ngokusebenzisa i-regio- kanye ne-stereoselective hydrolysis yohlu olubanzi lwe-aliphatic ne-aromatic nitriles.
Uhlobo lokusabela kwe-Catalytic:
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
| I-Enzyme Powder | ES-NIT-101~ ES-NIT-140 | isethi ye-40 Nitrilase, 50 mg ngayinye izinto ezingama-40 * 50mg / into, noma elinye inani |
| Ikhithi Yokuhlola (SynKit) | ES-NIT-4000 | isethi 40 Nitrilase, 1 mg ngayinye 40 izinto * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo se-buffer (i-pH yokusabela efanelekile) kanye ne-ES-NIT.
➢ Wonke ama-ES-NIT angahlolwa ngokulandelana kwawo ohlelweni lokusabela olungenhla noma nge-NIT Screening Kit (SynKit NIT).
➢ Zonke izinhlobo ze-ES-NIT ezihambisana nezimo zokusabela ezifanele kufanele zifundwe ngazodwana.
➢ I-Substrate egxilile ephezulu noma umkhiqizo onawo ungase uvimbele umsebenzi we-ES-NIT.Kodwa-ke, ukuvimbela kungakhululwa ngokungezwa kwe-batch ye-substrate.
Isibonelo 1(Ukwenziwa kwe-nicotinic acid)(1):
Isibonelo 2(Ukuhlanganiswa kwe-(R)-(-)-mandelic acid)(2):
Isibonelo 3(Ukwenziwa kwe-baclofen)(3):
Isibonelo 4(Ukwenziwa kwe-glycolic acid)(4):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1 Mathew CD, Nagasawa T, Kobayashi M, e tal.Uhlelo lokusebenza.Imvelo.I-Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal.Uhlelo lokusebenza.Imvelo.I-Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, e tal.Isilevu.J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, no tal.I-Biotechnol.I-Bioeng., 2008, 99(3): 717.
