I-Imine reductase (IRED)
Incazelo emfushane:
Mayelana ne-Imine reductase
I-ES-IRED: Uhlobo lwama-enzyme akhuthaza ukwehla kwesibopho se-C=N kusibopho se-CN. Lungokwe-enzyme ye-redox, ene-coenzyme NADPH yokuthutha i-hydrogen. Ngokwezintandokazi ze-chiral zemikhiqizo, zingahlukaniswa zibe yi-R-IRED kanye ne-S-IRED. Ama-Syncozymes athuthukise ama-imine reductases angu-15 (i-numbIRED ES-IRED-101-ES-IRED-115), angasetshenziswa kabanzi ekunciphiseni ama-imine ngokukhetha kanye nokukhetha ama-stereo ukuze kulungiselelwe ama-chiral amine.
Uhlobo lokusabela kwe-Catalytic:
Iselula/I-Wechat/I-WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | I-ES-IRED-101~ I-ES-IRED-114 | isethi ye-14 Imine Reductase, 50 mg ngayinye |
| Ikhithi Yokuhlola Ama-enzyme E-Well angu-96 | I-ES-IRED-1400 | isethi ye-14 Imine Reductase, 1 mg ngayinye |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-coenzyme, kanye nohlelo lokuvuselela i-coenzyme.
➢ Zonke izinhlobo ze-ES-IREDs ezihambisana nezimo ezahlukahlukene zokusabela ezifanele kufanele zifundwe ngazinye.
➢ I-substrate eningi noma umkhiqizo one-concentration ephezulu ungavimbela umsebenzi we-ES-IRED. Kodwa-ke, ukuvinjelwa kungancishiswa ngokufaka i-substrate ngenqwaba.
Isibonelo 1 (Ukwenziwa kwe-chiral 2-methyl pyrrolidine)(1):
Isibonelo 2 (Ukwenziwa kwe-amine yesibili)(2):
Isibonelo 3 (Ukunciphisa Ama-Cyclic Imines)(3):
Gcina iminyaka emibili ngaphansi kuka -20℃.
Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
1. Scheller PN, Fademrecht S, Hofelzer S, et al. I-ChemBioChem, 2014, 15, 2201-2204.
2. Wetzl D, Gand M, Ross A, nabanye. ChemCatChem, 2016, 8, 2023-2026.
3. U-Li H, uLuan ZJ, u-Zheng GW, et al. Adv. I-Synth. I-Catal. 2015, 357, 1692-1696.
