I-Ene reductase (ERED)
Ama-ES-ERED enza izinhlobo ezahlukene ze-substrate ngenxa yobuningi bama-substrate.Ngokuvamile, i-C=C ye-α, i-β -unsaturated compounds namaqembu a-electron-absorbing (okuhlanganisa i-ketone aldehyde, amaqembu e-nitro, i-carboxylic acids, i-esters, i-anhydride, i-lactones, imines, njll.) iyancipha kalula ngama-ES-EREDs, kodwa amabhondi aphindwe kabili angasebenzi.
Kunezinhlobo ezingu-46 zemikhiqizo ye-enzyme ye-ERED (Inombolo njenge-ES-ERED-101~ES-ERED-146) ethuthukiswe yi-SyncoZymes.
Indlela ye-Catalytic:
| Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
| I-Enzyme Powder | ES-ERED-101~ ES-ERED-146 | isethi ye-46 Ene Reductases, 50 mg ngayinye 46 izinto * 50mg / into, noma elinye inani |
| Ikhithi Yokuhlola (SynKit) | ES-ERED-4600 | isethi ye-46 Ene Reductases, 50 mg ngayinye 46 izinto * 50mg / into, noma elinye inani |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
★ ukuphepha okuphezulu.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isixazululo se-buffer (i-pH yokusabela kahle kakhulu), ama-coenzymes(NAD(H) noma i-NADP(H) ), uhlelo lokuvuselela i-coenzyme(isb i-glucose ne-glucose dehydrogenase) kanye ne-ES-ERED.
➢ Wonke ama-ES-ERED angahlolwa ngokulandelana kwawo ohlelweni lokusabela olungenhla noma nge-ERED Screening Kit (SynKit ERED).
➢ Zonke izinhlobo ze-ES-ERED ezihambisana nezimo zokusabela ezifanele kufanele zifundwe ngazodwana.
➢ I-substrate egxilile kakhulu noma umkhiqizo onawo ungase uvimbele umsebenzi we-ES-ERED.Kodwa-ke, ukuvimbela kungakhululwa ngokungezwa kwe-batch ye-substrate.
Isibonelo 1(α,β-unsaturated aldehydes noma ketones)(1):
Isibonelo 2(α,β-unsaturated carboxylic acid kanye nokuphuma kwayo)(2):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. Lucídio C, Fardelone J, Augusto R, e tal.J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, e tal..Org.Lett, 2007, 9(26): 5409-5411.
