I-Ene reductase (ERED)
Ama-ES-ERED akhuthaza izinhlobo ezahlukene ze-substrate ngenxa yobubanzi be-substrate. Ngokuvamile, i-C=C yama-α, β-unsaturated compounds anamaqembu athatha ama-electron (kufaka phakathi i-ketone aldehyde, amaqembu e-nitro, ama-carboxylic acid, ama-esters, i-anhydride, ama-lactone, ama-imines, njll.) ancishiswa kalula yi-ES-EREDs, kodwa ama-double bond angasebenzanga awancishiswa.
Kunezinhlobo ezingu-46 zemikhiqizo ye-enzyme ye-ERED (Inombolo njenge-ES-ERED-101~ES-ERED-146) eyathuthukiswa yi-SyncoZymes.
Indlela yokwenza izinto nge-catalytic:
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | ES-ERED-101~ ES-ERED-146 | isethi yama-Ene Reductase angu-46, angu-50 mg ngalinye izinto ezingu-46 * 50mg / into, noma olunye ubuningi |
| Ikhithi Yokuhlola (i-SynKit) | I-ES-ERED-4600 | isethi yama-Ene Reductase angu-46, angu-50 mg ngalinye izinto ezingu-46 * 50mg / into, noma olunye ubuningi |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
★ Ukuphepha okuphezulu.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer (i-pH yokusabela efanele), ama-coenzyme (i-NAD(H) noma i-NADP(H)), uhlelo lokuvuselela i-coenzyme (isib. i-glucose ne-glucose dehydrogenase) kanye ne-ES-ERED.
➢ Wonke ama-ES-ERED angahlolwa ngokulandelana ohlelweni lokusabela olungenhla noma nge-ERED Screening Kit (SynKit ERED).
➢ Zonke izinhlobo ze-ES-ERED ezihambisana nezimo ezahlukahlukene zokusabela ezifanele kufanele zifundwe ngazinye.
➢ I-substrate eningi noma umkhiqizo one-concentration ephezulu ungavimbela umsebenzi we-ES-ERED. Kodwa-ke, ukuvinjelwa kungancishiswa ngokufaka i-substrate ngenqwaba.
Isibonelo 1(α,β-unsaturated aldehydes noma ama-ketone)(1):
Isibonelo 2(α,β-unsaturated carboxylic acids kanye ne-derivatives yazo)(2):
Gcina iminyaka emibili ngaphansi kuka -20℃.
Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. UStueckler C, Hall M, Ehammer H, etal. .Org.Lett, 2007, 9(26): 5409-5411.
