I-Transaminase (ATA)
Ama-enzyme: Angama-catalyst ebhayoloji e-macromolecular, ama-enzyme amaningi angamaprotheni.
Ama-Transaminase: Uhlobo lwama-enzyme avuselela ama-amino adlulisa phakathi kwama-amino acid nama-keto acid. Ama-Transaminase angama-enzyme abalulekile ebhayoloji ekwakhiweni okungalingani kanye nokuxazululwa kwe-racemic kwama-chiral amines.
Ama-aminotransferase angahlukaniswa ngezigaba ezine ngokuya ngokulandelana kanye nesakhiwo: Ⅰ, Ⅱ, Ⅲ kanye no Ⅳ. Ama-ω-aminotransferase angaphansi kwama-transaminase ekilasi II, avame ukusetshenziswa ekulungiseleleni ama-chiral amines nama-amino acid angewona awemvelo, njenge-β-amino acids.
Ama-aminotransferase ka-ω: Ezimweni eziningi, i-ω-transaminase ibhekisela ekilasini lama-enzyme, lawo aguqula ukusabela kokudlulisa kwe-ammonia ngaphandle kwe-α-amino acid njenge-substrate noma umkhiqizo.
Indlela yokwenza izinto nge-catalytic:
| Ama-enzyme | Ikhodi Yomkhiqizo | Ikhodi Yomkhiqizo |
| Impuphu ye-Enzyme | I-ES-ATA-101~ ES-ATA-165 | isethi yama-65 ω-Transaminases, 50 mg ngayinye izinto ezingama-65 * 50mg / into, noma olunye ubuningi |
| Ikhithi Yokuhlola (i-SynKit) | I-ES-ATA-6500 | isethi yama-65 ω-Transaminases, 1 mg ngayinye Izinto ezingama-65 * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukusebenza kahle kokuguqulwa.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
➢ Ukuhlolwa kwama-enzyme kufanele kwenziwe kuma-substrate athile ngenxa yobukhulu be-substrate, bese kutholwa i-enzyme evuselela i-substrate eqondiwe ngomphumela omuhle kakhulu we-catalytic.
➢ Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-amino donor (njenge-amino acid kanye ne-1-phenyl ethylamine) noma i-receptor (njenge-keto acids), i-coenzyme (PLP), i-cosolvent (njenge-DMSO).
➢ I-ATA kufanele ifakwe ekugcineni ohlelweni lokusabela, ngemuva kokuthi i-pH kanye nokushisa sekulungisiwe esimweni sokusabela.
➢ Zonke izinhlobo ze-ATA zinezimo ezahlukene zokusabela ezingcono kakhulu, ngakho-ke ngayinye yazo kufanele ifundwe kabanzi ngayinye.
Isibonelo 1 (ukwenziwa kwe-Sitagliptin, ukwenziwa kwe-asymmetric)(1):
Isibonelo 2 (i-Mexiletine, Inhlanganisela yesisombululo se-kinetic kanye nokuhlanganiswa okungalingani)(2):
1 USavile CK, uJaney JM, uMundorff EC, nabanye. Isayensi, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Izincwadi ze-Organic, 2009,11(21):4810-4812.
