I-Transaminase (ATA)
Ama-Enzymes: Ama-macromolecular biological catalysts, ama-enzyme amaningi angamaprotheni.
Ama-Transaminase: Ikilasi lama-enzyme enza ukudluliselwa kwama-amino phakathi kwama-amino acid nama-keto acid.Ama-transaminase angama-enzyme abalulekile ebhayoloji ekuhlanganiseni kwe-asymmetric kanye nokuxazululwa kwe-racemic yama-chiral amines.
I-Aminotransferase ingahlukaniswa ngamakilasi amane ngokuya ngokulandelana nesakhiwo: Ⅰ, Ⅱ, Ⅲ kanye no-Ⅳ.Ama-ω-aminotransferase ayingxenye yama-transaminase ekilasi II, avame ukusetshenziswa ekulungiseni ama-chiral amines nama-amino acid angewona awemvelo, njengama-β-amino acid.
Ama-ω-aminotransferase: Ezimweni eziningi, i-ω-transaminase ibhekisela ekilasini lama-enzyme, lokho kusabela kokudluliswa kwe-ammonia okuyi-catalytic ngaphandle kwe-α-amino acid njenge-substrate noma umkhiqizo.
Indlela ye-Catalytic:
| Ama-enzyme | Ikhodi Yomkhiqizo | Ikhodi Yomkhiqizo |
| I-Enzyme Powder | ES-ATA-101~ ES-ATA-165 | isethi 65 ω-Transaminase, 50 mg ngayinye 65 izinto * 50mg / into, noma omunye ubuningi |
| Ikhithi Yokuhlola (SynKit) | ES-ATA-6500 | isethi 65 ω-Transaminase, 1 mg ngayinye 65 izinto * 1mg / into |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ High ukuguqulwa ukusebenza kahle.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ukuhlolwa kwe-enzyme kufanele kwenziwe kuma-substrate athile ngenxa yokucaciswa kwe-substrate, futhi uthole i-enzyme eyenza i-substrate eqondiwe ibe nomthelela omuhle kakhulu we-catalytic.
➢ Ungalokothi uthintane nezimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-organic solvent enokugxila okuphezulu.
➢ Ngokuvamile, isistimu yokusabela kufanele ihlanganise i-substrate, isisombululo se-buffer, amino donor (njengama-amino acids ne-1-phenyl ethylamine) noma i-receptor (njenge-keto acids), i-coenzyme (PLP), i-cosolvent (njenge-DMSO).
➢ I-ATA kufanele yengezwe okokugcina ohlelweni lokusabela, ngemuva kokuthi i-pH nezinga lokushisa kulungiswe esimweni sokusabela.
➢ Zonke izinhlobo ze-ATA zinemibandela ehlukahlukene yokusabela kahle, ngakho ngayinye yazo kufanele iqhutshekwe ifundwe ngayodwa.
Isibonelo 1(ukuhlanganiswa kwe-Sitagliptin, i-asymmetric synthesis)(1):
Isibonelo sesi-2 (i-Mexiletine, Inhlanganisela yokulungiswa kwe-kinetic ne-asymmetric synthesis)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.Isayensi, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Izincwadi ze-Organic, 2009,11(21):4810-4812.
