Isithombe Esifakiwe se-Transaldolase (TAL)

I-Transaldolase (TAL)

Incazelo emfushane:

Mayelana ne-Transaldolase

I-ES-TAL (i-Transaldolase): ikakhulukazi ibhekisela kuma-transaldolase e-threonine, angavuselela ukusabela kokwenziwa kwama-derivatives e-β-hydroxyphenylalanine nama-derivatives e-threonine ne-benzaldehyde. I-TAL iyi-enzyme encike ku-PLP. Kukhona uhlobo olulodwa kuphela lomkhiqizo we-enzyme ye-TAL (Inombolo njenge-ES-TAL101) eyathuthukiswa yi-SyncoZymes, engasetshenziswa ukwenziwa kwe-β-hydroxyphenylalanine kanye nama-derivatives ayo.

Uhlobo lokusabela kwe-Catalytic:

I-Transaldolase TAL

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-PLP, i-Mg²⁺.
➢ I-TAL kufanele ifakwe ekugcineni ohlelweni lokusabela, ngemuva kokuthi i-pH kanye nokushisa sekulungisiwe esimweni sokusabela.

Izibonelo Zokusetshenziswa:

Isibonelo 1 (Ukwenziwa kwe-(2S, 3R)-p-methylsulfonyl phenylserine kusuka ku-p-methylsulfonyl benzaldehyde kanye ne-threonine)⁽¹⁾:

Isitoreji:

Gcina iminyaka emibili ngaphansi kuka -20℃.

Ukunakwa:

Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.