I-Transaldolase (TAL)
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okuqinile kwe-chiral.
★ ukuguqulwa okuphezulu.
★ Imikhiqizo engaphansi.
★ Izimo zokusabela ezithambile.
★ Ayinandaba nemvelo.
➢ Ngokuvamile, isistimu yokusabela kufanele ifake i-substrate, isisombululo se-buffer, i-enzyme, i-PLP, i-Mg2+.
➢ I-TAL kufanele yengezwe okokugcina ohlelweni lokusabela, ngemva kokuthi i-pH nezinga lokushisa kulungiswe esimweni sokusabela.
Isibonelo 1(Ukwenziwa kwe-(2S, 3R)-p-methylsulfonyl phenylserine kusuka ku-p-methylsulfonyl benzaldehyde kanye ne-threonine)(1):
Gcina iminyaka emi-2 ngaphansi -20 ℃.
Ungalokothi uthintane nezimo eziyingozi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye ne-solvent ephilayo egxilile.
1. CN109836362B
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