Isithombe Esifakiwe se-Nitrile reductase (NRED)

I-Nitrile reductase (NRED)

Incazelo emfushane:

Mayelana ne-Nitrile reductase

Kunezinhlobo ezintathu zomkhiqizo we-enzyme ye-NRED (Inombolo njenge-ES-NRED101~ ES-NRED103) eyathuthukiswa yi-SyncoZymes. I-NRED ingakhuthaza ukwehla kweqembu le-nitrile libe yi-amine. Enkambisweni ye-catalysis, i-NADPH iyadingeka njengomthuthi we-hydrogen.

Uhlobo lokusabela lwe-Nitrile reductasecatalytic

I-Nitrile reductase NRED2

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Imininingwane
Imininingwane	ES- NRED -101~ ES- NRED -103	isethi yama-Nitrile Reductase ama-3, ama-50 mg ngalinye izinto ezi-3 * 50mg / into, noma olunye ubuningi

Inqubo yokuhlola ukusabela kwe-Nitrile reductase:

Uhlelo lokusabela olulandelayo lungasetshenziswa njengereferensi:
I-substrate engu-1-10 mg/ml
I-enzyme engu-10 mg/ml
10% (v/v) I-cosolvent yemvelo (1)
I-100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
I-glucose engu-10 mg/ml
2 mg/ml I-Glucose dehydrogenase (GDH)
Faka ukusabela ku-temperature shaker engaguquki (isibonelo, 30oC, 150rpm) amahora angama-24 ~ 48. Faka i-centrifuge ingxube ngayinye ukuze i-protein isheshe, bese i-supernatant ingasetshenziswa ekuhlaziyeni kwe-TLC, HPLC noma i-GC.
Qaphela (1): Isinyibilikisi se-organic (njenge-DMSO, i-methanol, i-acetonitrile, i-IPA, njll.) singakhethwa, sisetshenziselwa izinto ezinganyibiliki.