I-oocyte iyisiqalo sempilo yomuntu, iyiqanda elingakavuthwa eligcina livuthiwe libe yiqanda. Kodwa-ke, ikhwalithi ye-oocyte iyancipha njengoba abesifazane bekhula noma ngenxa yezici ezifana nokukhuluphala ngokweqile, futhi ama-oocyte asezingeni eliphansi yimbangela eyinhloko yokuzala okuphansi kwabesifazane abakhuluphele. Kodwa-ke, indlela yokuthuthukisa ikhwalithi yama-oocyte kwabesifazane abakhuluphele kuyinselele kososayensi.
Muva nje, ucwaningo olunesihloko esithi Ukuphathwa kwe-nicotinamide mononucleotide kuthuthukisa ikhwalithi yamagundane akhuluphele lushicilelwe ku-Cell proliferation. Ucwaningo luthole ukuthi ukwengezwa kwe-nicotinamide adenine dinucleotide (NAD+) precursori-nicotinamide mononucleosideI-Acid (i-NMN) ingathuthukisa ngempumelelo ukuvuvukala kwesibeletho, ithuthukise ikhwalithi ye-oocyte futhi ibuyisele isisindo somzimba sabantwana emagundwini esifazane akhuluphele.
Abacwaningi bakhethe amagundane esifazane anamasonto amathathu ubudala kanye namagundane esilisa anamasonto ayi-11 ubudala ukuze basungule imodeli yegundane yokukhuluphala ngokudla okunamafutha amaningi futhi kwaqinisekiswa ngokuqopha isisindo, ukuhlolwa kweglucose egazini ngokuzila ukudla, kanye nokuhlolwa kokubekezelela ushukela ngomlomo, ukungenelela kokudla 1 Kwamasonto ama-2,I-NMNIzithasiselo zajovwa izinsuku eziyi-10 zilandelana ukuze kutholakale ukubonakaliswa kwezakhi zofuzo ezihlobene nokukhula kwe-ovarian kanye nezakhi zofuzo ezihlobene nokuvuvukala, ubukhulu bamafutha ezicubu zamafutha esiswini, izinga lezinhlobo ze-oxygen ezisabelayo zama-oocyte, isakhiwo se-spindlechromosome, Umsebenzi we-mitochondrial, i-actin dynamics, kanye nomonakalo we-DNA, uma kuqhathaniswa nemiphumela yezibalo ekhombisa:
1. Imodeli yegundane equkethe ukudla okunamafutha amaningi yasungulwa ngempumelelo
Inani le-FGB leqembu lokudla okunamafutha amaningi (HFD) laliphakeme njalo kuneqembu lokudla okuvamile (ND), ngaphezu kwalokho, imiphumela ye-OGTT ibonise ukuthi amagundane eqembu lokudla okunamafutha amaningi (HFD) ayengabekezeleli ushukela.
2. I-NMN ingathuthukisa ukungasebenzi kahle kwe-metabolic kumagundane e-HFD
Ukwengezwa ngeI-NMNukwengezwa kwezakhi zofuzo kunciphisa ivolumu yamafutha emagundwini eqenjini lokudla okunamafutha amaningi (HFD), futhi imiphumela iphakamisa ukuthi i-NMN inomphumela wokuvikela ekusetshenzisweni kwe-metabolism okungavamile emagundwini eqenjini lokudla okunamafutha amaningi (HFD).
3. I-NMN ithuthukisa ikhwalithi yama-ovari kumagundane e-HFD
I-NMN ingathuthukisa ikhwalithi yama-ovari kumagundane anamafutha amaningi (i-HFD) ngokulawula ukuvezwa kwezakhi zofuzo ezibalulekile ezihlobene nokuthuthukiswa kwe-follicle yama-ovari (i-Bmp4, i-Lhx8) kanye nokuvuvukala.
4. I-NMN inciphisa ukukhubazeka kokuhlukaniswa kwama-oocyte kanye nomonakalo we-DNA kumagundane e-HFD
I-NMN inganciphisa imvamisa ephezulu yeziphambeko ze-spindle kanye nokungahambisani kahle kwama-chromosome okubangelwa ukudla okunamafutha amaningi (HFD), inciphise ukusayinwa kwe-γH2A.X, futhi ithuthukise umonakalo we-DNA obangelwa ukudla okunamafutha amaningi (HFD) ngokunciphisa ukuvezwa kwe-Bax.
5. I-NMN ingathuthukisa ikhwalithi yama-oocyte
I-NMN ingalungisa ukubonakaliswa kwe-antioxidant SOD1 okuphansi eqenjini lokudla okunamafutha amaningi (i-HFD), ithuthukise ukusatshalaliswa kwe-mitochondria, futhi ingathuthukisa ikhwalithi yama-oocyte ngokugcina ubuqotho be-cytoskeleton.
6. I-NMN ingabuyisela ukusatshalaliswa kwamaconsi e-lipid kumagundane e-HFD
Ama-oocyte eqembu lokudla okunamafutha amaningi (HFD) ayephezulu kancane kunalawo asema-oocyte eqembu lokudla okuvamile (ND), futhi ukwengezwa kwe-NMN kunganciphisa ukuqina kwe-fluorescence kwamaconsi e-lipid.
7. I-NMN ibuyisela isisindo somzimba ebantwaneni bamagundane e-HFD
Isisindo sokuzalwa senzalo yeqembu lokudla okunamafutha amaningi (i-HFD) sasiphansi kakhulu kuneseqembu lokudla okuvamile (i-ND), futhi ukwengezwa nge-NMN kwandisa isisindo sokuzalwa senzalo yeqembu le-HFD.
Kulolu cwaningo, abacwaningi babonise ngokuhlolwa kwamagundane ukuthi i-NMN inekhono lokuthuthukisa ikhwalithi yama-oocyte kumagundane abesifazane abakhuluphele okubangelwa ukudla okunamafutha amaningi, futhi baveze ukuthi i-NMN ingabuyisela ukusebenza kwe-mitochondrial futhi inciphise ukuqongelela kwe-ROS kuma-oocyte amagundane abesifazane abakhuluphele. , umonakalo we-DNA kanye nezindlela eziyisisekelo zokusatshalaliswa kwamaconsi e-lipid. Ngakho-ke, lolu cwaningo luzohlinzeka isu lokwelapha elingase libe khona lokuthuthukisa izinkinga zokuzala ezibangelwa ukukhuluphala kwabesifazane.
izinkomba:
1.Wang L, Chen Y, Wei J, nabanye. Ukuphathwa kwe-nicotinamide mononucleotide kuthuthukisa ikhwalithi yama-oocyte amagundane akhuluphele. Cell Prolif. 2022; e13303. doi10. 1111cpr.13303
Isikhathi sokuthunyelwe: Septhemba-28-2022