I-oocyte iyisiqalo sempilo yomuntu, iyingqamuzana leqanda elingavuthiwe eligcina livuthwe libe iqanda.Nokho, ikhwalithi ye-oocyte iyehla njengoba abesifazane bekhula noma ngenxa yezinto ezifana nokukhuluphala ngokweqile, futhi ama-oocyte ekhwalithi ephansi ayimbangela eyinhloko yokuzala okuphansi kwabesifazane abakhuluphele.Nokho, indlela yokuthuthukisa izinga lama-oocyte kwabesifazane abakhuluphele kuyinselele kososayensi.
Muva nje, ucwaningo olunesihloko esithi Administration of nicotinamide mononucleotide luthuthukisa ikhwalithi ye-oocyte yamagundane akhuluphele lushicilelwe ku-Cell proliferation.Ucwaningo luthole ukuthi ukwengezwa kwe-nicotinamide adenine dinucleotide (NAD+) isandulela.I-nicotinamide mononucleosideI-Acid (NMN) ingathuthukisa ngempumelelo ukuvuvukala kwe-ovarian, ithuthukise ikhwalithi ye-oocyte futhi ibuyisele isisindo somzimba wengane kumagundane abesifazane abakhuluphele .
Abacwaningi bakhethe amagundane esifazane anamaviki angu-3 ubudala kanye namagundane wesilisa anesonto le-11 ukuze kusungulwe imodeli yegundane lokukhuluphala ngokudla okunamafutha amaningi futhi kuqinisekiswe ukuqoshwa kwesisindo, ukuhlolwa kweglucose yegazi okusheshayo, nokuhlolwa kokubekezelela i-glucose ngomlomo, ukungenelela kokudla. 1 Amasonto ama-2,NMNizithasiselo zajovwa izinsuku eziyi-10 ezilandelanayo ukuze kutholwe ukuvezwa kwezakhi zofuzo ezihlobene nokukhula kwe-ovarian kanye nezakhi zofuzo ezihlobene nokuvuvukala, usayizi wamafutha wezicubu ze-adipose esiswini, izinga lezinhlobo zomoya-mpilo osebenzayo wama-oocyte, isakhiwo se-spindlechromosome, Umsebenzi we-mitochondrial, amandla e-actin, nokulimala kwe-DNA , uma kuqhathaniswa nemiphumela yezibalo ekhombisayo:
1. Imodeli yegundane yokudla okunamafutha amaningi -okubangelwa ukukhuluphala yasungulwa ngempumelelo
Inani le-FGB leqembu le-high-fat diet (HFD) laliphakeme ngokuqhubekayo kuneqembu elivamile lokudla ( ND ), ngaphezu kwalokho, imiphumela ye-OGTT ibonise ukuthi amagundane eqembu lokudla okunamafutha aphezulu ( HFD ) ayengabekezeleli i-glucose.
2. I-NMN ingathuthukisa ukungajwayelekile kwe-metabolic kumagundane e-HFD
Ukwengeza ngeNMNukwesekwa kuncishiswe umthamo wamafutha kumagundane eqenjini lokudla okunamafutha aphezulu ( HFD ), futhi imiphumela ibonisa ukuthi i-NMN inomphumela ovikelayo kumetabolism engavamile kumagundane eqenjini lokudla okunamafutha aphezulu ( HFD ) .
3. I-NMN ithuthukisa ikhwalithi yesibeletho kumagundane e-HFD
I-NMN ingathuthukisa ikhwalithi ye-ovarian ekudleni okunamafutha aphezulu (i-HFD) amagundane ngokulawula ukubonakaliswa kwezakhi zofuzo ezibalulekile ezihlobene nokuthuthukiswa kwe-ovarian follicle (Bmp4, Lhx8) nokuvuvukala.
4. I-NMN yehlisa amaphutha e-oocyte division kanye nokulimala kwe-DNA kumagundane e-HFD
I-NMN inganciphisa imvamisa ephezulu yokukhubazeka kwe-spindle kanye nokungahambi kahle kwe-chromosomal okubangelwa ukudla okunamafutha aphezulu ( HFD ), ukunciphisa ukusayinda kwe-γH2A.X, nokunciphisa ukudla okunamafutha aphezulu ( HFD ) -okubangelwa ukulimala kwe-DNA ngokunciphisa inkulumo ye-Bax.
5. I-NMN ingathuthukisa ikhwalithi yama-oocyte
I-NMN ingalungisa inkulumo elawulwa phansi ye-antioxidant SOD1 eqenjini lokudla okunamafutha aphezulu (i-HFD), ithuthukise ukusatshalaliswa kwe-mitochondria, futhi ingathuthukisa ikhwalithi yama-oocyte ngokugcina ubuqotho be-cytoskeleton.
6. I-NMN ingabuyisela ukusatshalaliswa kwe-lipid droplet kumagundane e-HFD
ukudla okunamafutha aphezulu ( HFD ) ama-oocyte eqembu ayephakeme kancane kunalawo okudla okuvamile ( ND ) ama-oocyte eqembu, futhi ukwesekwa kwe-NMN kunganciphisa amandla e-fluorescence amaconsi e-lipid.
7. I-NMN ibuyisela isisindo somzimba enzalweni yamagundane e-HFD
isisindo sokuzalwa senzalo yeqembu lokudla okunamafutha aphezulu ( HFD ) sasiphansi kakhulu kuneqembu lokudla okuvamile ( ND ), futhi ukuxhaswa nge-NMN supplementation kwabuyisela isisindo sokuzalwa senzalo yeqembu le-HFD.
Kulolu cwaningo, abacwaningi babonise ngokuhlolwa kwegundane ukuthi i-NMN inamandla okuthuthukisa ikhwalithi yama-oocyte kumagundane abesifazane abakhuluphele abangelwe ukudla okunamafutha amaningi, futhi yembula ukuthi i-NMN ingabuyisela ukusebenza kwe-mitochondrial futhi yehlise ukunqwabelana kwe-ROS kuma-oocyte egundane labesifazane abakhuluphele., ukulimala kwe-DNA kanye nezindlela eziyisisekelo zokusatshalaliswa kwamaconsi e-lipid.Ngakho-ke, lolu cwaningo luzohlinzeka ngesu lokwelapha elingase libe khona lokuthuthukisa izinkinga zokuzala ezibangelwa ukukhuluphala kwabesifazane .
izinkomba:
1.Wang L, Chen Y, Wei J, et al.Ukuphathwa kwe-nicotinamide mononucleotide kuthuthukisa ikhwalithi ye-oocyte yamagundane akhuluphele.Iseli Prolif.2022;e13303.doi10.1111cpr.13303
Isikhathi sokuthumela: Sep-28-2022