I-glucose dehydrogenase (GDH)
Incazelo emfushane:
Mayelana ne-Glucose dehydrogenase
I-ES-GDH (i-glucose dehydrogenase): I-GDH ivuselela ukukhishwa kwe-glucose ukuze ikhiqize i-gluconic acid (i-lactone), futhi yehlise i-NAD(P)+ ibe yi-NAD(P)H, nge-NAD(P)+ njenge-electron receptor. I-GDH ivame ukusetshenziselwa ukuvuselelwa kwe-coenzyme NAD(P)H emikhakheni ye-biocatalysis nezinye i-enzyme eyinhloko. Kunezinhlobo eziyi-10 zemikhiqizo ye-enzyme ye-GDH (ES-GDH-101~ES-GDH-110) eyenziwe yi-SyncoZymes, phakathi kwazo i-ES-GDH110 enomsebenzi ophezulu kakhulu futhi iyi-GDH enconywe kakhulu.
Uhlobo lokusabela kwe-Catalytic:
Iselula/I-Wechat/I-WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | ES-GDH-101~ ES-GDH-109 | Ama-enzyme ayi-9*50mg/pc, noma olunye ubuningi |
★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.
➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-enzyme, kanye ne-coenzyme.
➢ Uma i-GDH isetshenziselwa ukuvuselelwa kwe-coenzyme, i-enzyme eyinhloko iyadingeka, futhi uhlelo lokusabela kufanele lwakhiwe ngokwe-enzyme eyinhloko.
Isibonelo 1 (Ukwenziwa kwe-biocatalysis kwe-imine kuya ku-chiral amine nge-imine reductase)(1):
Gcina iminyaka emibili ngaphansi kuka -20℃.
Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.
1. Bernhard LM, McLachlan J, Gröger H. Ukuthuthukiswa Kwenqubo Ye-Enantioselective Imine Reductase-Catalyzed Syntheses of Pharmaceutically Relevant Pyrrolidines [J]. Ucwaningo Nokuthuthukiswa Kwenqubo Yemvelo, 2022.
