Isithombe Esifakiwe se-Carboxylic acid reductase (CAR)

I-Carboxylic acid reductase (CAR)

Incazelo emfushane:

Mayelana ne-Carboxylic acid reductase

I-ES-CAR (i-Carboxylic acid reductase): ivuselela ukwehla kweqembu le-carboxyl libe yiqembu le-aldehyde. Enqubweni yokuvuselela, ukusebenza kwe-ATP kanye ne-coenzyme NADPH kuyadingeka njengabathuthi be-hydrogen. Kunezinhlobo ezimbili zemikhiqizo ye-enzyme ye-CAR (Inombolo njenge-ES-CAR101~ ES-CAR-102) eyenziwe yi-SyncoZymes.

Uhlobo lokusabela kwe-Catalytic:

I-Carboxylic acid reductase (CAR)1

Iselula/I-Wechat/I-WhatsApp: +86-13681683526

I-imeyili:lchen@syncozymes.com

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ulwazi Lomkhiqizo:

Ama-enzyme	Ikhodi Yomkhiqizo	Imininingwane
Ikhithi Yokuhlola (i-SynKit)	I-ES-CAR-101-ES-CAR-102	isethi yama-Ketoreductase amabili, 1 mg ngayinye Izinto ezi-2 * 1mg/into

Izinzuzo:

★ Ukucaciswa okuphezulu kwe-substrate.
★ Ukukhetha okunamandla kwe-chiral.
★ Ukuguqulwa okuphezulu.
★ Imikhiqizo embalwa engaphansi.
★ Izimo zokusabela okuncane.
★ Inobungani nemvelo.

Imiyalelo yokusebenzisa:

➢ Ngokuvamile, uhlelo lokusabela kufanele luhlanganise i-substrate, isixazululo se-buffer, i-enzyme, i-coenzyme, i-ATP.
➢ I-CAR kufanele ifakwe ekugcineni ohlelweni lokusabela, ngemuva kokuthi i-pH kanye nokushisa sekulungisiwe esimweni sokusabela.

Izibonelo Zokusetshenziswa:

Isibonelo 1 (Ukwenziwa kwe-cinnamyl alcohol kusuka ku-phenylalanine)⁽¹⁾:

Isitoreji:

Gcina iminyaka emibili ngaphansi kuka -20℃.

Ukunakwa:

Ungalokothi uthinte izimo ezimbi kakhulu ezifana nalezi: izinga lokushisa eliphezulu, i-pH ephezulu/ephansi kanye nesinyibilikisi se-organic esinomthamo ophezulu.