Ucwaningo Express |Ucwaningo lweNyuvesi yaseTsinghua lukhombisa ukuthi i-NMN ingakwelapha ukuvuvukala

Ukusebenza kwe-Macrophage kuyindlela ye-pathogenic eholela ekuvuvukeni okungapheli emzimbeni , kodwa ukusebenza okuqhubekayo kwe-macrophage kungabangela ukuvuvukala okungapheli kanye nezifo ezifana nokumelana ne-insulin kanye nezifo ezinzima ezifana ne-atherosclerosis.I-PGE 2, ehlanganisa impendulo yokuvuvukala, ihlanganiswa ne-arachidonic acid ngama-cyclooxygenases (COX-1 ne-COX-2).I-COX-1 ne-COX-2 yizona ezihlosiwe eziyinhloko zokulwa nokuvuvukala futhi zingavinjelwa izidakamizwa ezingezona ukuvuvukala (ama-NSAID).

ukusetshenziswa kwama - NSAID kungabangela ukusabela okubi okuningi , njengokuphuma kwamathumbu emathunjini .Ngakho-ke, kubaluleke kakhulu ukuthola into ephephile yemvelo yokwelapha ukuvuvukala .

Muva nje, ithimba labacwaningi abavela eNyuvesi yaseTsinghua belaphe ama-macrophages egundane nge-NMN, futhi lafakazela ngokuhlolwa ukuthi i-NMN inganciphisa ukunqwabelana kwamaprotheni ahlobene nokuvuvukala kanye nemikhiqizo ye-metabolic, futhi ivimbele ukusabela kokuvuvukala kwama-macrophages.Imingcele ku-Molecular Biosciences.

Ukuvuvukala kushintsha amazinga we-metabolic byproducts kuma-macrophage
Okokuqala, ithimba locwaningo lenze ama-macrophages asebenze ukuze akhiqize ukuvuvukala nge-lipopolysaccharide ( LPS ) , lase lihlaziya okuqukethwe kwemikhiqizo ezungeze ama-macrophage ngesikhathi sokuvuvukala.Amazinga wama-metabolites angama-99 anyuswa futhi ama-metabolite ayi-105 ehla phakathi kwama-molecule angama-458 atholwe ngaphambi nangemva kokugqugquzelwa kokuvuvukala, futhi amazinga e-NAD+ ahambisana nokuvuvukala nawo ancipha.

(UMFANEKISO 1)

I-NMN inyusa amazinga e-NAD futhi inciphisa ukuvuvukala kwe-macrophage
Ithimba labacwaningi labe seliphatha ama-macrophages nge-LPS, eyenza isimo sokuvuvukala, i-IL-6 ne-IL-1β, ama-cytokines abangela ukuvuvukala asebenza njengezimpawu zokuvuvukala .Ngemuva kokwelashwa kwe-NMN yokuvuvukala kwe-macrophage eyenziwe yi-LPS, kutholakale ukuthi izinga le-NAD le-intracellular lanyuswa futhi inkulumo ye-mRNA ye-IL-6 ne-IL-1β yehlisiwe.Ukuhlola kubonise ukuthi i-NMN ikhuphule izinga le-NAD kanye nokuvuvukala kwe-macrophage okubangelwa yi-LPS okuncishisiwe .

(Umfanekiso 2)

(Umfanekiso 3)

I-NMN inciphisa amazinga amaprotheni ahlobene nokuvuvukala
Ukwelashwa kwe-NMN, kutholakale ukuthi amaprotheni ahlobene nokuvuvukala okufana ne-RELL1, i-PTGS2, i-FGA, i-FGB ne-igkv12-44 yancipha kumaseli, okubonisa ukuthi i-NMN inciphisa ukubonakaliswa kwamaprotheni ahlobene nokuvuvukala.

(Umfanekiso 4)

I-NMN inciphisa ukuvezwa kwamaprotheni ahlosiwe we-NSAIDS
Ukuhlolwa kokugcina kuthole ukuthi i-NMN inciphise izinga le-PGE2 kumaseli e-RAW264.7 acushwe yi-LPS ngokunciphisa izinga lokubonisa le-COX-2, ngaleyo ndlela yehlisa ukubonakaliswa kwe-COX2 nokuvimbela ukuvuvukala okubangelwa i-LPS.

(Umfanekiso 6)

isiphetho , ukwesekwa kwe-NMN kungaphatha ngokuphumelelayo ukuvuvukala okungapheli kumagundane, futhi ukwelashwa kokuvuvukala kubantu kusadinga ukuqinisekiswa ngokuhlolwa komtholampilo okufanelekile.Mhlawumbe i-NMN izoba esikhundleni se-NSAIDS maduze nje.

izinkomba:
1.Liu J, Zong Z, Zhang W, Chen Y, Wang X, Shen J, Yang C, Liu X, Deng H. I-Nicotinamide Mononucleotide Inciphisa Ukuvuvukala Okubangelwa I-LPS Nokucindezeleka Oxidative Ngokunciphisa Ukuvezwa kwe-COX-2 kuma-Macrophages.Ngaphambili Mol Biosci.2021 Julayi 6.