I-Research Express | Ucwaningo lwe-Tsinghua University lubonisa ukuthi i-NMN ingalapha ukuvuvukala

Ukusebenza kwe-macrophage kuyindlela ebangela izifo eholela ekuvuvukeni okungapheli emzimbeni, kodwa ukusebenza kwe-macrophage okuqhubekayo kungaholela ekuvuvukeni okungapheli kanye nezifo ezifana nokumelana ne-insulin kanye nezifo ezinzima ezifana ne-atherosclerosis. I-PGE 2, elawula impendulo yokuvuvukala, yenziwa kusuka ku-arachidonic acid yi-cyclooxygenases (COX-1 kanye ne-COX-2). I-COX-1 kanye ne-COX-2 yizona zinhloso eziyinhloko zokulwa nokuvuvukala futhi zingavinjelwa yimithi engeyona i-steroidal yokulwa nokuvuvukala (ama-NSAID).

Ukusetshenziswa kwama-NSAID kungabangela ukusabela okuningi okubi, njengokuphuma kwegazi esiswini. Ngakho-ke, kubaluleke kakhulu ukuthola into yemvelo ephephile yokwelapha ukuvuvukala.

Muva nje, ithimba locwaningo elivela eNyuvesi yaseTsinghua lelaphe ama-macrophage egundane nge-NMN, futhi lafakazela ngokuhlola ukuthi i-NMN inganciphisa ukuqongelela kwamaprotheni ahlobene nokuvuvukala kanye nemikhiqizo ye-metabolic, futhi ivimbele impendulo yokuvuvukala kwama-macrophage. Imingcele ku-Molecular Biosciences.

Ukuvuvukala kushintsha amazinga emikhiqizo elandelanayo ye-metabolic kuma-macrophage
Okokuqala, ithimba locwaningo lenze kwasebenza ama-macrophage ukuze akhiqize ukuvuvukala nge-lipopolysaccharide (LPS), bese lihlaziya okuqukethwe yimikhiqizo ezungeze ama-macrophage ngesikhathi sokuvuvukala. Amazinga ama-metabolite angu-99 akhushulwa kanti ama-metabolite angu-105 ancishiswa phakathi kwama-molecule angu-458 atholwe ngaphambi nangemva kokuvuselelwa kokuvuvukala, kanti amazinga e-NAD+ ahambisana nokuvuvukala nawo ancishisiwe.

(UMFANEKISO 1)

I-NMN inyusa amazinga e-NAD futhi inciphise ukuvuvukala kwe-macrophage
Ithimba locwaningo labe seliphatha ama-macrophage nge-LPS, okubangela isimo sokuvuvukala, i-IL-6 kanye ne-IL-1β, ama-cytokine akhuthaza ukuvuvukala asebenza njengezimpawu zokuvuvukala. Ngemva kokwelashwa kwe-NMN kokuvuvukala kwe-macrophage okubangelwa yi-LPS, kwatholakala ukuthi izinga le-NAD langaphakathi kweseli landa futhi ukubonakaliswa kwe-mRNA kwe-IL-6 kanye ne-IL-1β kwehle. Ukuhlolwa kubonise ukuthi i-NMN yandisa izinga le-NAD futhi yanciphisa ukuvuvukala kwe-macrophage okubangelwa yi-LPS.

(Isithombe 2)

(Isithombe 3)

I-NMN yehlisa amazinga amaprotheni ahlobene nokuvuvukala
Ukwelashwa kwe-NMN, kwatholakala ukuthi amaprotheni ahlobene nokuvuvukala njenge-RELL1, PTGS2, FGA, FGB kanye ne-igkv12-44 ancishisiwe kumaseli, okubonisa ukuthi i-NMN yehlisa ukubonakaliswa kwamaprotheni ahlobene nokuvuvukala.

(Isithombe 4)

I-NMN inciphisa ukuvezwa kwamaprotheni aqondiwe e-NSAIDS
Ukuhlolwa kokugcina kuthole ukuthi i-NMN yehlise izinga le-PGE2 kumaseli e-RAW264.7 asebenza nge-LPS ngokunciphisa izinga lokubonakaliswa kwe-COX-2, ngaleyo ndlela kunciphisa ukubonakaliswa kwe-COX2 futhi kuvimbele ukuvuvukala okubangelwa yi-LPS.

(Isithombe 6)

Isiphetho, ukwengezwa kwe-NMN kungalapha ngempumelelo ukuvuvukala okungapheli emagundwini, futhi ukwelashwa kokuvuvukala kubantu kusadinga ukuqinisekiswa yizivivinyo zemitholampilo ezifanele. Mhlawumbe i-NMN izoba indawo ye-NSAIDS maduze nje.

izinkomba:
I-1.Liu J, Zong Z, Zhang W, Chen Y, Wang X, Shen J, Yang C, Liu X, Deng H. I-Nicotinamide Mononucleotide Inciphisa Ukuvuvukala Okubangelwa I-LPS Nokucindezeleka Kwe-Oxidative Ngokunciphisa Ukuvezwa kwe-COX-2 kuma-Macrophages. Ngaphambili Mol Biosci. 2021 Julayi 6.