I-Leucine Dehydrogenase (LeuDH)
Incazelo emfushane:
Mayelana ne-Leucine dehydrogenase
Kukhona uhlobo olulodwa lomkhiqizo we-enzyme ye-LeuDH (Inombolo njenge-ES-LeuDH-101) eyakhiwa yi-SyncoZymes. I-LeuDH ingavuselela i-ammoniation enciphisa i-4-methylbutanoic acid (usawoti) ibe yi-L-leucine noma i-oxidation ye-leucine ibe yi-4-methylbutanoic acid. I-ES-LeuDH ingavuselela futhi i-amination enciphisa i-α-keto acid enesakhiwo esifanayo ukulungiselela ama-amino acid derivatives ahambisanayo. Enkambisweni ye-catalysis, i-NADH iyadingeka njengomthuthi we-hydrogen.
Uhlobo lokusabela kwe-Leucine dehydrogenase
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I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Imininingwane |
| Impuphu ye-Enzyme | I-ES-LeuDH-101 | isethi ye-1 Monoamine Oxidase, 50 mg ngayinye Into e-1 * 50mg / into, noma olunye ubuningi |
Uhlelo lokusabela olulandelayo lungasetshenziswa njengereferensi:
1-10 mg/ml: I-Substrate
10 mg/ml: I-enzyme
10% (v/v): I-cosolvent yemvelo (1)
100 mM: I-Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: i-Ammonium formate
10 mg/ml: I-Formate dehydrogenase (FDH)
Faka ukusabela ku-temperature shaker engaguquki (isibonelo, 30oC, 150rpm) amahora angama-24 ~ 48. Faka i-centrifuge ingxube ngayinye ukuze i-protein isheshe, bese i-supernatant ingasetshenziswa ekuhlaziyeni i-TLC, i-HPLC noma i-GC.
Qaphela (1): Isinyibilikisi se-organic (njenge-DMSO, i-methanol, i-acetonitrile, i-IPA, njll.) singakhethwa, sisetshenziselwa izinto ezinganyibiliki.
