I-Leucine Dehydrogenase (LeuDH)
Incazelo emfushane:
Mayelana ne-Leucine dehydrogenase
Kunohlobo olungu-1 lomkhiqizo we-enzyme ye-LeuDH (Inombolo njengokuthi ES-LeuDH-101) eyakhiwe i-SyncoZymes.I-LeuDH ingakwazi ukuvuselela i-ammoniation enciphisa i-4-methylbutanoic acid (usawoti) ku-L-leucine noma i-oxidation ye-leucine ku-4-methylbutanoic acid.I-ES-LeuDH ingase futhi ibangele ukukhishwa okunciphisayo kwama-α-keto acid anesakhiwo esifanayo ukuze kulungiselelwe okuphuma kokuphuma kwe-amino acid okuhambisanayo.Enqubweni ye-catalysis, i-NADH iyadingeka njengesithuthi se-hydrogen.
Uhlobo lokusabela lwe-Leucine dehydrogenasecatalytic
Iselula/Wechat/WhatsApp: +86-13681683526
I-imeyili:lchen@syncozymes.com
| Ama-enzyme | Ikhodi Yomkhiqizo | Ukucaciswa |
| I-Enzyme Powder | ES-LeuDH-101 | isethi ye-1 Monoamine Oxidase, 50 mg into eyodwa 1 * 50mg / into, noma elinye inani |
Isistimu yokusabela elandelayo ingasetshenziswa njengereferensi:
1-10 mg/ml: I-Substrate
10 mg/ml: I-enzyme
10% (v/v): I-Organic cosolvent (1)
100 mM: Ibhafa ye-Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: I-Ammonium Formate
10 mg/ml: Yenza i-dehydrogenase (FDH)
Fukamisa ukusabela endaweni enyakazisa izinga lokushisa engashintshi (isibonelo, 30oC, 150rpm) amahora angama-24~48.Faka i-Centrifuge ingxube ngayinye ukuze uthole amaprotheni, wona amandla angaphezu kwamandla angasetshenziselwa ukuhlaziywa kwe-TLC, HPLC noma i-GC.
Qaphela (1): Isinyibilikisi se-Organic (njenge-DMSO, i-methanol, i-acetonitrile, i-IPA, njll.) iyakhethwa, isetshenziselwa ama-substrates angancibiliki.
